Clones have been constructed that contain full-length cDNA copies of cowpea mosaic virus RNA1 and RNA2, downstream of the cauliflower mosaic virus 35S promoter. The clones, when linearized downstream of the viral sequences, give rise to cowpea mosaic virus-like symptoms when inoculated onto cowpea plants. Viral RNA and virions can be detected in the inoculated plants, demonstrating that the clo...

Full-length cDNAs of barley mild mosaic bymovirus RNA1 and RNA2 were cloned downstream of a modified cauliflower mosaic virus 35S promoter with double enhancer. Mechanical inoculation of barley seedlings with a mixture of both cDNAs resulted in systemic mosaic symptoms, typical of barley mild mosaic virus infection. The presence of both RNA species and their gene products in the systemically in...

Two bipartite begomoviruses, Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV), have been isolated from mosaic-diseased cassava originating from central India and Sri Lanka, respectively. ICMV was transmitted with low efficiency from cassava to Nicotiana benthamiana by sap inoculation to give leaf curl symptoms. SLCMV was much more virulent in this host, producing s...

Hfstorfcd Uses for Celery Celery has figured as a natural medicine in different cultures throughout recorded history. And since the midtwentieth century, scientists have been testing the allergenic components of celery and related vegetables. Most Americans think of celery as nothing more than a relatively harmless vegetable. It is also used primarily by vegetarians as a source of fiber and nat...

The developed duplex real-time PCR method allows the simultaneous detection of traces of potentially allergenic white mustard (Sinapis alba) and celery roots (Apium graveolens var. rapaceum), celery stalks (A. g. var. dulce) and leaf celery (A. g. var. secalinum). The duplex assay does not show any cross-reactivity with 64 different biological species, including various members of the Brassicac...

in recent years, symptoms of a viral disease including streak mosaic, leaf blade malformation, and thickness (scrawniness) of raceme have been observed in the vast areas of grain fields in golestan province. in order to identify the causes of this disease, detection of wheat streak mosaic virus (wsmv) in cultivated hosts including wheat, dominant weeds of fields involving barnyardgrass (echinoc...