An adipic acid esterification experiment suitable for graduate students has been developed to describe the industrial manufacture of plasticizers. Students first follow progress reaction with simple acid/base titration enabling measurement number. During workup mixture, they learn how manage filtration a viscous mixture over an inert support such as Celite and practice on atmospheric vacuum dis...

The flocculation tests currently used for the serodiagnosis of syphilis involve the use of an aqueous emulsion of cholesterol, beef heart lecithin, and cardiolipin which undergoes aggregation when treated with Wassermann antibody (1). The r81e of cholesterol in ihis reaction has been depicted as providing a particulate surface which enhances the visibility of the aggregates formed by the antige...

Two trials of similar experimental design were conducted to assess the effects of various dietary enzymes on energy digestibility of diets high in distillers dried grains with solubles (DDGS) for broilers. A 2 × 4 factorial arrangement of treatments was used in which a cornsoybean meal control diet and a diet with 30% DDGS were supplemented with 2 different commercial enzyme products. In additi...

Abstract Lipase is an enzyme commonly applied as a catalyst in the food industry, synthesis of bioplastics, and production biodiesel. Candida rugosa lipase (CRL) one widely used lipases due to its high selectivity activity. However, operating temperatures can denature enzymes, affecting activity structural changes. An be immobilized on supporting matrix improve stability. Ethanol immobilization...

Abstract Flavanones have been prepared by the cyclization of 2'‐hydroxychalcones using acidic reagents, such as H 2 SO 4 , HX, CF 3 COOH, CH H, KF‐celite, or SiO . Treatment with bases, LiOH, KOH, NaOAc, K CO pyridine, Et N, N ‐methylimidazole afforded flavanones. The conversion to flavanones has conducted Co(salpr), Fe(CN) 6 CuO, Ni(Tfacac) Thioflavanones deprotecting and cyclizing S ‐protecte...

Treatment of pea (Pisum sativum L.) hypocotyl segments with indole-3-butyric acid, which promotes segment elongation, increased the solubilization of both xyloglucan and cello-oligosaccharides in the apoplast of auxin-treated pea stems. The cello-oligosaccharides were isolated from the apoplastic solution with a charcoal/Celite column and were identified as cellobiose, cellotriose, and cellotet...

With the use of real-time PCR, we developed and evaluated a rapid, sensitive, specific, and reproducible method for the detection of Epstein-Barr virus (EBV) DNA in plasma samples. This method allowed us to screen plasma and serum samples over a range between 100 and 10(7) copies of DNA per ml using two sample preparation methods based on absorption. A precision study yielded an average coeffic...

An experiment was conducted with laying hens to determine the AME(n) value of crude glycerol, a coproduct of biodiesel production. Crude glycerol (87% glycerol, 9% water, 0.03% methanol, 1.26% Na, and 3,625 kcal/kg of gross energy) was obtained from a commercial biodiesel production facility (Ag Processing Inc., Sergeant Bluff, IA). A total of forty-eight 40-wk-old laying hens (Hy-Line W-36) we...

Ceramic passive samplers or toximeters (packed with active carbon 1%, w/w, on celite), in combination with the CALUX bioassay have been used as a time-integrated monitoring technique for dissolved dioxin-like PCBs in urban and industrial wastewaters. The technique showed to be reliable during laboratory experiments: (1) PCB-126 amounts extracted from the passive samplers increased linearly with...