Anti-infective drug development depends to a great extent on enrolling the “correct” subjects in Ph II–III clinical trials – i.e., those who can show a therapeutic benefit from the anti-infective therapy. For example, while at Lederle Labs, I worked on a new indication for MINOCIN®-namely, treatment of moderate-to-severe periodontitis. MINOCIN®-reduces the numbers of the putative periodontal pa...

The recent advent of high-throughput approaches has revealed widespread transcription of the human genome, leading to a new appreciation of transcription regulation, especially from noncoding regions. Distinct from most coding and small noncoding RNAs, long noncoding RNAs (lncRNAs) are generally expressed at low levels, are less conserved and lack protein-coding capacity. These intrinsic featur...

Most common techniques for spatialization require the listener to be positioned at a “sweet spot” surrounded by loudspeakers. For practical concert, stage, and installation applications such layouts may not be desirable. Distance-based amplitude panning (DBAP) offers an alternative panningbased spatialization method where no assumptions are made concerning the layout of the speaker array nor th...

Biopanning has been used extensively in conjunction with purified components, but there are also examples in which mixtures of targets have been investigated. This study introduces a methodological innovation, termed iterative panning and blocking (IPAB), to extend the range of specific interactions that can be probed in mixtures. Here this procedure is used to probe a mixture of high molecular...

The city of Bou Sa?da is one the Algerian cities that have diverse tourism resources, but it has not been adopted as a premier tourist spot nor benefited from programs established within national strategy for sustainable development Horizons 2030, which made weak compared to other regions similar elements. This study aims evaluate existing address shortcomings and provide solutions by focusing ...

Ecotin, a dimeric serine protease inhibitor from Escherichia coli, is a novel platform for inhibitor design. An approach using the three-dimensional structure of the ecotin-trypsin complex to guide combinatiorial design efforts was taken to create potent bidentate ecotin inhibitors for trypsin and human urokinase-type plasminogen activator (uPA). The ecotin surface loop that was redesigned is c...

Long insertions into a loop of a folded host protein are expected to have destabilizing effects because of the entropic cost associated with loop closure unless the inserted sequence adopts a folded structure with amino- and carboxy-termini in close proximity. A loop entropy reduction screen based on this concept was used in an attempt to retrieve folded sequences from random sequence libraries...

This study compares the stage metaphor and the channel strip metaphor in terms of performance. Traditionally, music mixing consoles employ a channels strip control metaphor for adjusting parameters such as volume and panning of each track. An alternative control metaphor, the so-called stage metaphor lets the user adjust volume and panning by positioning tracks relative to a virtual listening p...