The objective of the study was to evaluate thecytotoxic effects of aqueous fruit extract of Cucumis melo in human prostate cancer cell line (PC-3) using MTTand neutral red assays. The crude aqueous extract of C.melo was prepared by cold maceration method, filtered,concentrated and tested on PC-3 cell line. Dose-dependent cytotoxic activitieswere exhibited by human prostate carcinoma PC-3 cell l...

Immunotherapy has been used for years in many types of cancer therapy. Recently, cancer immunotherapy has focused on mechanisms which can enhance the development of cell-mediated immunity. Anticancer medications are administered to inhibit immunosuppressive factors such as nagalase enzyme, which is produced by neoplastic cells and destroys macrophage activating factor (Gc-MAF). Anti-neoplastics...

background: erythropoietin (epo) is a glycoprotein hormone function to regulate the production of red blood cells. deficiency of epo is known to cause anemia in chronically infected renal patients and they require regular blood transfusion. availability of recombinant epo has eliminated the need for blood transfusion and now it is extensively used for the treatment of anemia. glycosylation of e...

recent findings in stem cell biology have opened a new window in regenerative medicine. the endometrium possesses mesenchymal stem cells (mscs) called endometrial stem cells (enscs) having specific regenerative properties linked to adult stem cells. they contribute in tissue remodeling and engineering and were shown to have immuno-modulating effects. many clinical trials were undertaken to asce...

Recent findings in stem cell biology have opened a new window in regenerative medicine. The endometrium possesses mesenchymal stem cells (MSCs) called endometrial stem cells (EnSCs) having specific regenerative properties linked to adult stem cells. They contribute in tissue remodeling and engineering and were shown to have immuno-modulating effects. Many clinical trials were undertaken to asce...

nucleostemin (ns), a stem cell-abundant nucleolar protein, is critical for maintaining the self-renewal and proliferative properties of normal and cancerous stem cells. recent data suggests that ns signaling is important for proliferation of t-cells and leukemia cells. this study was conducted to verify the role of ns in pathogenesis and treatment of t-cell acute lymphocytic leukemia (t-all). o...

background: autophagy suppression recently has been known to have a remarkable effect for cellular adjustment and viability in the final stages of cancer. on the other hand, autophagy has the potential effect in preventing many viruses from replication. beclin1 is the most substantial constituent in autophagy apparatus regulation. this study was intended to investigate the beclin1 sirna knockdo...

background glioblastoma is a type of brain tumor with poor response to available therapies, and shows high rate of mortality. despite remarkable advancements in our knowledge about cytogenetic and pathophysiologic features of glioblastoma, current treatment strategies are mainly based on cytotoxic drugs; however, these therapeutic approaches are facing progressive failure because of the resista...

Stem cells are self-renewing cells that can be differentiated into other cell types. Conventional in vitro models for studying stem cells differentiation are usually preformed in two-dimensional (2D) cultures. The design of three-dimensional (3D) in vitro models which ideally are supposed to mimic the in vivo stem cells microenvironment is potentially useful for inducing stem cell derived tissu...

This paper reviews recent strategies used for increasing specific yield and productivity in high cell density cultures. High cell density cultures offer an efficient means for the economical production of recombinant proteins. However, there are still some challenges associated with high cell density cultivation (HCDC) techniques. A variety of strategies in several aspects including host design...