نتایج جستجو برای: cell selex
تعداد نتایج: 1684463 فیلتر نتایج به سال:
In vitro experiments that characterize DNA-protein interactions by artificial selection, such as SELEX,are often performed with the assumption that the experimental conditions are equivalent to natural ones. To test whether SELEX gives natural results, we compared sequence logos composed from naturally occurring leucine-responsive regulatory protein (Lrp) binding sites with those composed from ...
SELEX (systematic evolution of ligands by exponential enrichment) is a very powerful method for determining the binding site of a protein on RNA. It relies on the ability of an RNA-binding protein to select high-affinity RNA ligands from a randomized pool of RNAs. SELEX experiments are performed over several "rounds," with each round resulting in increased enrichment of RNAs capable of binding ...
Aptamer is a protein-binding oligonucleotide which can be in vitro selected by systematic evolution of ligands by exponential enrichment (SELEX), and it has the advantages of high affinity, low immunogenicity and easy synthesis as a promising class of antibody surrogates. The microfluidic SELEX performs an improved multiplex platform which can run a number of aptamer selection in parallel and s...
Aptamers are a class of therapeutic oligonucleotides that form specific three-dimensional structures that are dictated by their sequences. They are typically generated by an iterative screening process of complex nucleic acid libraries employing a process termed Systemic Evolution of Ligands by Exponential Enrichment (SELEX). SELEX has traditionally been performed using purified proteins, and c...
Tumor necrosis factor-alpha (TNFα) is a multifunctional cytokine associated with inflammation, immune responses, and autoimmune diseases including rheumatoid arthritis, inflammatory bowel disease, psoriasis. In the present study, we performed in vitro selection, systematic evolution of ligands by exponential enrichment (SELEX) against human TNFα from mRNA-displayed peptide library prepared Esch...
SELEX-Seq is now the optimal high-throughput technique for characterizing DNA-binding specificities of transcription factors. In this study, we introduced an improved EMSA-based SELEX-Seq strategy with several advantages. The improvements of this strategy included: (1) using a FAM-labeled probe to track protein-DNA complex in polyacrylamide gel for rapidly recovering the protein-bound dsDNA wit...
The RNA-binding protein Roquin is required to prevent autoimmunity. Roquin controls T-helper cell activation and differentiation by limiting the induced expression of costimulatory receptors such as tumor necrosis factor receptor superfamily 4 (Tnfrs4 or Ox40). A constitutive decay element (CDE) with a characteristic triloop hairpin was previously shown to be recognized by Roquin. Here we use S...
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