A method for the preparation of HeLa cell plasma membrane ghosts is described. The purity of the plasma membrane fraction was examined by phase contrast and electron microscopy, by chemical analysis, and by assay of marker enzymes. Data on the composition of the plasma membrane fraction are given. It was observed that the distribution pattern of 5'-nucleotidase activity among the subcellular fr...

Recombinant immunoconjugates of marker enzymes with antigens or antibodies present considerably more advantages than those obtained by conventional methods of chemical synthesis; i.e. they are homogeneous, have a strictly determined stoichiometry, and retain the functional activity of both a marker protein and an antigen/antibody. Based on the pPICZαB shuttle vector, we first managed to obtain ...

HPLC, column and thin layer chromatography guided studies led to the isolation of seven different compounds in methanolic extracts of Podophyllum hexandrum. The isolated compounds were analyzed using LC-MS and High Performance Liquid Chromatography (HPLC) studies interfaced to mass spectroscopy. Isolated compounds were used successfully as chemical markers for the comparison of the twelve diffe...

Bystin (BYSL) is a 306-amino acid protein encoded in humans by the BYSL gene located on the 6p21.1 chromosome. It is conserved across a wide range of eukaryotes. BYSL was reported to be a sensitive marker for the reactive astrocytes induced by ischemia/reperfusion and chemical hypoxia in vitro and is considered to be one of the common characteristics of astrogliosis. In our study we examined wh...

This Perspective highlights recent advances in infrared vibrational chemical nano-imaging. In its implementations of scattering scanning near-field optical microscopy (s-SNOM) and photothermal-induced resonance (PTIR), IR nanospectroscopy provides few-nanometer spatial resolution for the investigation of polymer, biomaterial, and related soft-matter surfaces and nanostructures. Broad-band IR s-...

Discovery of molecular markers for efficient identification of living organisms remains a challenge of high interest. The diversity of species can now be observed in details with low cost genomic sequences produced by new generation of sequencers. A method, called c-GAMMA, is proposed. It formalizes the design of new markers for such data. It is based on a series of filters on forbidden pairs o...

In this paper, we describe a novel algorithm to group, label, identify and perform optical tracking of marker sets, which are grouped into two specific configurations, and whose projective invariant properties will allow obtaining a unique identification for each predefined marker pattern. These configurations are formed by 4 collinear and 5 coplanar markers. This unique identification is used ...

This fall marks 20 years since the cloning of unc-119 was reported. Despite having a strong phenotype that makes animals somewhat difficult to grow and handle, unc-119 mutant rescue has become one of the most frequentlyused markers for C. elegans transformation. In this Commentary, I describe the history of how unc-119 rescue traveled through the worm community, contributing to the development ...

This paper provides two puzzles for a theory of aspect. The first concerns the quirky behavior of the Russian imperfective with regard to its culmination properties: it seems to function like the perfect aspect in certain cases, but like the progressive in others. The other puzzle concerns how the Russian imperfective constrains the temporal location of a described event: it relates distinct ev...