The effect of glucose deprivation on the synthesis of lipid-linked oligosaccharides by Chinese hamster ovary cells has been studied. When these cells are placed in serum-free Dulbecco's minimal essential medium devoid of glucose, there is a rapid cessation of the synthesis of the usual Glc3Man9GlcNAc2 lipid-linked oligosaccharide and the accumulation of a smaller species with the composition Ma...

Chinese hamster ovary cells show endogenous high-affinity Na(+)-dependent glutamate transport activity. This transport activity is kinetically similar to a glutamate transporter family strategically expressed in the central nervous system and is pharmacologically unlike glutamate transporter-1 or excitatory amino acid carrier 1. The cDNA of a glutamate/aspartate transporter (GLAST)-like transpo...

facilitates apoptosis by targeting several anti-apoptotic genes. If miR-466h-5p activation is demonstrated to be a universal early response in apoptotic pathways in CHO, 2 Mü ller, D. et al. (2008) MicroRNAs as targets for engineering of CHO cell factories. Trends Biotechnol. 26, 359–365 3 Xu, X. et al. (2011) The genomic sequence of the Chinese hamster ovar (CHO)-K1 cell line. Nat. Biotechnol....

We have created a genome-scale network reconstruction of chinese hamster ovary (CHO) cell metabolism. Existing reconstructions were improved in terms of annotation standards, to facilitate their subsequent use in dynamic modelling. The resultant network is available from ChoNet (http://cho.sf.net/).

We investigated if carbohydrate (CHO) availability could affect the excess post-exercise oxygen consumption (EPOC) after a single supramaximal exercise bout. Five physically active men cycled at 115% of peak oxygen uptake (V̇O2 peak) until exhaustion with low or high pre-exercise CHO availability. The endogenous CHO stores were manipulated by performing a glycogen-depletion exercise protocol 48 ...

BACKGROUND It is recommended that endurance athletes consume carbohydrate (CHO) supplements, providing 6-8% CHO concentration, during exercise > 60 minutes to improve athletic performance. Recently research has compared carbohydrate-protein (CHO-P) supplementation to the traditionally used CHO supplementation during endurance exercise, following these supplementation recommendations, in control...

The object of the present paper is to derive a property of certain meromorphic functions in the punctured unit disk. Our main theorem contains certain sufficient conditions for starlikeness and close-to-convexity of order α of meromorphic functions. 2000 Mathematics Subject Classification. 30C45.

To determine whether the relative utilization of exogenous carbohydrate (CHO(exo)) differs between children and adults, substrate utilization during 60 min of cycling at 70% peak O(2) uptake was studied in 12 pre- and early pubertal boys (9.8 +/- 0.1 yr) and 10 men (22.1 +/- 0.5 yr) on two occasions. Subjects consumed either a placebo or a (13)C-enriched 6% CHO(exo) beverage (total volume per t...

This application note describes how the Agilent Bio-Monolith Protein A column was applied to the optimization of Chinese hamster ovary cell-culture conditions to produce a recombinant monoclonal antibody with desired structural characteristics, focusing on glycosylation. The workflow demonstrates the versatility and wide applicability of the column in biopharmaceutical and biosimilar development.

In recent years, the number of recombinant proteins used for therapeutic applications and industry has increased dramatically. Recombinant proteins are produced in many host organisms (microbial, insect, plant and mammalian cells). There are many factors to consider when choosing the optimal system for protein expression and purification including the mass, purity or solubility of the recombina...