This paper reports a methodology for fast analysis of two important antioxidants: tyrosol and oleuropein in real samples of alimentary oils by using a ready-to-use portable microfluidic system (MicruX ® iHVStat) based on microchips electrophoresis with electrochemical detection. The portable system integrates for the first time a high voltage power supply and a bipotentiostat for using microflu...

A monolithic capillary electrophoresis system with integrated on-chip fluorescence detector has been microfabricated on a silicon substrate. Photodiodes in the silicon substrate measure fluorescence emitted from eluting molecules. The device incorporates an on-chip thin-film interference filter that prevents excitation light from inhibiting the fluorescence detection. A transparent AZO conducti...

Sorting and sizing of DNA molecules by capillary electrophoresis (CE) within the human genome project have enabled the genetic mapping of various illnesses. By the use of miniaturized lab-on-achip devices, on-chip-integrated DNA sequencing and genetic diagnostics have become feasible. The inherent advantages of microfluidic CE separation of differently sized DNA molecules, high-speed operation ...

Microfluidics chip technology is a powerful and convenient alternative to agarose gels and PAGE, but costs can be high due to certain chips being non-reusable. Here we describe a method to regenerate, re-use, and store Agilent DNA, RNA, and protein electrophoresis chips designed for use in the Bioanalyzer 2100. By washing the sample wells and displacing the old gel matrix with new gel-dye mix, ...

We developed a novel single-step capillary electrophoresis (SSCE) scheme for field-amplified sample stacking (FASS) by using a hydrophilic material polymethyl methacrylate (PMMA) microchannel chip. The capillary effect was used to introduce liquids and control capillary stop valves in a partial barrier structure of the microchannel. Through the combined action of stop valves and air vents, both...

We present a novel labeling technique for detection and speciation of organochloride compounds using microcapillary zone electrophoresis coupled with laser induced fluorescence detection. Organochloride compounds in an aqueous mixture are conjugated with glutathione using 1,5 Diazabicyclo [4.3.0] non-5-ene as a catalyst. The conjugated species are then labeled with Pacific Blue succinimidyl est...

This paper presents a systematic investigation of the viability of using internal standards to correct for chip-to-chip imprecision in disposable microchip capillary electrophoresis (μCE) devices. The effects of two internal standards (CsCl and LiCl) on the reproducibility of Na quantification were explored using inhouse fabricated microchips. Five concentrations of NaCl were analyzed consecuti...

Inkjet 3D printing is with success used for preparation microfluidics devices as a microfluidic chips for DNA separation. Utilized this technic help us to prepare a chip in shorter time and cheaper, but length of microchannel are limited by possibility of support material removing. One of the Solution for solve this limitations is divide a microfluidics chips for modules and printing them separ...

1 Abstract— A rapid and cheap method has been developed for the fabrication of a capillary electrophoresis chip for the preliminary characterization of particles. The microfluidic chips were fabricated using polymethyl methacrylate (PMMA) with integrated platinum electrodes without the need of using high technology microfabrication techniques. The chips were characterized using electroosmotic f...

The development of an on-animal separation-based sensor that can be employed for monitoring drug metabolism in a freely roaming sheep is described. The system consists of microdialysis sampling coupled to microchip electrophoresis with electrochemical detection (MD-ME-EC). Separations were accomplished using an all-glass chip with integrated platinum working and reference electrodes. Discrete s...