On the tropical legume Sesbania rostrata, stem-borne nodules develop after inoculation of adventitious root primordia with the microsymbiont Azorhizobium caulinodans. A cDNA clone, Srchi13, with homology to acidic class III chitinase genes, corresponds to an early nodulin gene with transiently induced expression during nodule ontogeny. Srchi13 transcripts accumulated strongly 2 days after inocu...

Antifreeze proteins similar to two different chitinases accumulate during cold acclimation in winter rye (Secale cereale). To determine whether these cold-responsive chitinases require post-translational modification to bind to ice, cDNAs coding for two different full-length chitinases were isolated from a cDNA library produced from cold-acclimated winter rye leaves. CHT9 is a 1,193-bp clone th...

The gene encoding a major, inducible 45 kDa chitinase of Aspergillus fumigatus was cloned and analysis of the deduced amino acid sequence identified a chitinase of the fungal/bacterial class which was designated ChiB1. Recombinant ChiB1, expressed in Pichia pastoris, was shown to function by a retaining mechanism of action. That is, the beta-conformation of the chitin substrate linkage was pres...

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下顎前突症は比較的多 くみ られ る不正咬合を主徴 とす る顎骨変形症の1つ で,そ の治療法 と して矯正的方法 と 口腔外科的方法,あ るいは両者を併用す る方法が ある。 外科的治療は,顔 貌 および咬合状態 の改善,下 顎全体 と しての機能回復な どを 目的 として行われ,そ の術式には 下顎枝両側を切断 し下顎骨体を後方へ移動 させ る方法, および両側下顎骨々体 の1部 を切除短縮す る方法 とに大 別される1)2).下顎枝切断術にはそ の切断部位に よって種 々の方法があ り,大 別す ると水平切断法3)4),斜 断法5)~8), 垂直切断法9),矢 状切断法10)~14),などが ある.水 平切断 法は古 くか ら行わ れて き た方法で,Kostecka3)が これ らを改良 し,Kostecka氏 法 として報告 してい る.そ の 後,わ が国では中村15)に...

treatment planning poses difficulties in edentulous patients for orthognathic surgery prediction and fixation.treatment of severe class iii malocclusion frequently requires orthognathic surgical procedures. for such patients, orthognathic surgery would be the only option before prosthetic rehabilitation.this clinical report describes step-by-step fabrication of a surgical splint for an edentulo...

Protein extracts from soybean (Glycine max [L.] Merr) seed hulls were fractionated by isoelectric focusing and SDS-PAGE analysis and components identified by peptide microsequencing. An abundant 32 kDa protein possessed an N-terminal cysteine-rich hevein domain present in class I chitinases and in other chitin-binding proteins. The protein could be purified from seed coats by single step bindin...