(CAA)-affected vessel in the Tg2576 mouse model of amyloid deposition. Representative example of CAA in a vessel segment of a living Tg2576 mouse imaged with multiphoton microscopy. Vascular and parenchymal A deposits are identified by fluorescence from systemically administered methoxy-XO4 (red pseudocolor). Fluorescent angiograms with Texas Red dextran were performed to identify fiduciary mar...

The structure of a 23 nt RNA sequence, rGGACCCGGGCUCAACCUGGGUCC, was elucidated using homonuclear NMR, distance geometry and restrained molecular dynamics. This RNA is analogous to residues 612-628 of the Escherichia coli 16S rRNA. The structure of the RNA reveals the presence of a pentaloop closed by a duplex stem in typical A-form conformation. The loop does not form a U-turn motif, as previo...

Coronary artery anomalies (CAAs) are a diverse group of congenital disorders whose manifestations and pathophysiological mechanisms are highly variable. The subject of CAAs is undergoing profound evolutionary changes related to the definition, morphogenesis, clinical presentation, diagnostic workup, prognosis, and treatment of these anomalies. To understand the clinical impact of CAAs, the fund...

This report forms, in two parts, deliverable D2.14 of the TurboNoiseCFD project. It describes the recommended innovative “triple plane pressure” in-duct matching strategy (TPP) at inlet and bypass duct side, and it describes in detail the newly developed and favourably tested CAA code for the propagation and radiation problem at the exhaust side. Due to the presence of the jet, this solution is...

Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showed that the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape...

In a previous study, we observed that the elimination clearance of 4-hydroxycyclophosphamide (HCY) in patients receiving cyclophosphamide (CY) 60 mg/kg/day by 1-h i.v. infusion for 2 consecutive days decreased from day 1 to day 2 due to an apparent decrease in human aldehyde dehydrogenase 1 (ALDH1) activity. Here, the mechanism for the decrease in ALDH1 activity after CY administration was inve...

Structural distortions on the boundary between right-handed B and left-handed Z DNA segments in plasmid pRW751 (a derivative of pBR322 containing (dC-dG)13 and (dC-dG)16 segments) were studied by means of chemical probes. Samples of supercoiled DNA were treated with the respective chemical probe, linearized with EcoRI and inhibition of BamHI (whose recognition sequence GGATCC lies on the bounda...

By using cytoplasmic and mitochondrial serine transhydroxymethylase isoenzymes from rabbit liver, it was shown that both enzymes exhibited similar ratios of serine transhydroxymethylase/threonine aldolase activities. Both enzymes catalysed the removal of the pro-S hydrogen atom of glycine, which was greatly enhanced by the presence of tetrahydrofolate. The cytoplasmic as well as the mitochondri...

hibitors used to treat human immunodeficiency virus (HIV)1. Tenofovir can be administered once-daily at a dose of 300 mg in combination with other antiretroviral drugs. The advantage of once-daily dosing is a reduction in patient drug load. Also, tenofovir appeared to be well tolerated with low rates of drug-related therapy discontinuation in several clinical trials. Thus, tenofovir use is expe...