Chlorophyll b is an ubiquitous accessory pigment in land plants, green algae, and prochlorophytes. Its biosynthesis plays a key role in the adaptation to various light environments. We isolated six chlorophyll b-less mutants by insertional mutagenesis by using the nitrate reductase or argininosuccinate lyase genes as tags and examined the rearrangement of mutant genomes. We found that an overla...

The light harvesting like protein 3 (LIL 3) from higher plants, has been linked to functions in chlorophyll and tocopherol biosynthesis, photo-protection and chlorophyll transfer. However, the binding of chlorophyll to LIL3 is unclear. We present a reconstitution protocol for chlorophyll binding to LIL3 in DDM micelles. It is shown in the absence of lipids and carotenoids that reconstitution of...

17-day-old bean plants (Phaseolus vulgaris cv. Strike) were used to analyze the effects of Co2+ and Zn2+ on the time course of proline, total protein, chlorophyll and abscisic acid (ABA) levels in leaves. Controls, Co2+ and Zn2+-treated plants were grown for 8 days in Hoagland solution. Samples were taken at 2 day intervals. Proline, chlorophyll (a+b) and total protein contents of 17 day old pr...

The partition of phylloquinone (vitamin K J, of chlorophylls a and b and of the two main carotenoids, /?-carotene and lutein, in subthylakoid particles (digitonin treatment) and chlorophyll protein complexes (sodium dodecylsulfate polyamide-gel electrophoresis) isolated from tobacco chloroplasts (Nicotiana tabacum L.) is described. 1. The “light particle” fractions (S 90000, S 150000) of digito...

Using high-intensity actinic light, the chlorophyll a fluorescence transient from HC0,-depleted chloroplasts shows a rapid initial rise (0 + 1) followed by a slow phase ( I + P). In the presence of HC0,-, the 0 -+ I rise is delayed but the I + P phase is much more rapid. Using lowintensity actinic light, the chlorophyll a fluorescence transient from 3-(3.4-dichlorophenyI)I , I dimethylurea (DCM...

Plants acclimate to variations in light intensity by changing the antenna size of photosystems. This acclimation allows them to undergo efficient photosynthesis and creates a protective strategy to minimize photodamage. Chlorophyll b synthesis by chlorophyllide a oxygenase (CAO) is a key regulatory step in the control of antenna size. Recently, we found that higher plant CAOs consist of three d...

In vitro culture of pericarp segments from fruit of Citrus sinensis (L.) Osbeck cv Valencia was used to determine the temporal sequence in development of chloroplasts from chromoplasts during regreening of the epicarp. Regreening of chromoplasts closely resembled greening of etioplasts, except that regreening proceeded much more slowly. Chlorophyll, the light-harvesting chlorophyll a/b binding ...

Representative plants containing either the reductive pentose phosphate cycle or the C(4) dicarboxylic acid cycle of photosynthetic carbon dioxide fixation have distinctly different contents of P(700) and chlorophylls a and b. With leaf extracts and isolated chloroplasts from C(4) cycle plants, the mean value of the relative ratio of P(700) to total chlorophyll was 1.83 and the mean value of th...

Introduction Leaf senescence involves degradation of proteins, The activities of chlorophyllase, contents of pigments chlorophylls, nucleic acids, membranes, and subsequent including chlorophyll a and b, chlorophyllide a and b, transport of some of the degradation products to other and phaeophorbide a during leaf senescence under parts of the plant (Buchanan-Wollaston, 1997; Noodén, low oxygen ...