This study investigates the ability of human plasma-derived lipid transfer protein to facilitate lipid transfer to and from intact viable cells in culture. Mouse peritoneal macrophages or J774 macrophages were preincubated with acetylated low density lipoprotein and [3H]oleate/albumin to promote the intracellular synthesis and accumulation of cholesteryl [3H]oleate and 3H-labeled triglyceride. ...

We describe here an activable neutral cholesteryl esterase (EC 3.1.1.13) in arteries similar to the hormone-sensitive lipase of adipose tissue and adrenal cortex. Maximum enzyme activity in rabbit aorta was given by cholesteryl ester substrates dispersed as a mixed micelle with phosphatidylcholine and Na taurocholate (molar ratio 1:4:2). A quantitative assay of enzymic activity was obtained wit...

The measurement of cholesteryl esters in human monocyte-derived macrophages using previously described high performance liquid chromatography methods is hampered by the presence in these cells of large amounts of triglycerides. We present a simple reversed phase high performance liquid chromatography protocol for quantification of cholesterol and cholesteryl esters in human monocyte/macrophages...

This article reviews aspects of the molecular pathology of cholesteryl ester accumulation in atherosclerotic lesions. 1. Transcytosis of lipoproteins through cultured endothelial monolayer. 2. Transformation to foam cell from macrophage. 3. Cholesteryl ester deposition in the extracellular space of atherosclerotic lesions. We also discuss the development and use of novel monoclonal antibodies r...

Rhesus monkeys were fed high cholesterol and cholesterol-free diets for 21-24 months. The animals were then given isotopic cholesterol intravenously and autopsied from 1 to 51 weeks later. The plasma and aortic cholesterol contents were 633 +/- 130 mg/dl and 35.6 +/- 11.4 mg/g dried tissue (45.5% in ester form) for atherosclerotic monkeys and 135 +/- 25 mg/dl and 9.9 +/- 3.6 mg/g (15.0% in este...

We have previously observed that cholesterol-fed dogs with plasma cholesterol levels of 350 to 750 mg/dl failed to develop atherosclerosis (hyporesponders), whereas cholesterol-fed dogs with cholesterol levels greater than 750 mg/dl developed markedly accelerated atherosclerosis (hyperresponders). Two striking features of the hypercholesterolemia of the hyperresponders were the occurrence of ch...

background: cholesteryl ester transfer protein (cetp) plays a main role in high-density lipoprotein metabolism. cetp gene possesses several single nucleotide polymorphisms which have been associated with plasma high-density lipoprotein cholesterol (hdl-c) concentrations. the aim of this study was to determine the association of cetp -629c/a and i405v polymorphisms with coronary artery disease (...

Normal human plasma HDL was applied to a column of heparin-Sepharose in the presence of MnCl(2) and three fractions were obtained by stepwise elution with increasing NaCl concentrations: a non-retained fraction (NR, 78% of protein) and two retained fractions (R(1) and R(2), 18 and 2.5% of protein, respectively). Both unesterified and esterified cholesterol increased from NR to R(1) to R(2) but ...

We have previously observed that cholesterol-fed dogs with plasma cholesterol levels of 350 to 750 mg/dl failed to develop atherosclerosis (hyporesponders), whereas cholesterol-fed dogs with cholesterol levels greater than 750 mg/dl developed markedly accelerated atherosclerosis (hyperresponders). Two striking features of the hypercholesterolemia of the hyperresponders were the occurrence of ch...

Hormone-sensitive lipase (HSL) contributes importantly to the hydrolysis of cholesteryl ester in steroidogenic tissues, releasing the cholesterol required for adrenal steroidogenesis. HSL has broad substrate specificity, because it hydrolyzes triacylglycerols (TAGs), diacylglycerols, monoacylglycerols, and cholesteryl esters. In this study, we developed a specific cholesterol esterase assay usi...