Warsaw breakage syndrome (WABS) is caused by defective DDX11, a DNA helicase that is essential for chromatid cohesion. Here, a paired genome-wide siRNA screen in patient-derived cell lines reveals that WABS cells do not tolerate partial depletion of individual APC/C subunits or the spindle checkpoint inhibitor p31(comet). A combination of reduced cohesion and impaired APC/C function also leads ...

This paper describes a two-dimensional computer simulation study of the impact induced breakage of brittle solid particles. The work started as an attempt to understand the effect of impact velocity on the induced breakage and in particular to explain why there is a shift in the slope of the size distribution at large impact velocities. The results indicate that the observed breakage pattern is...

in this study the wavelet neural network (wnn) and artificial neural network (ann) were used to simulate barley breakage percentage in combine harvester. the models have been trained using the same data conditions. air temperature, thresher cylinder speed, distance between thresher cylinder and concave (back and forth) and the percentage of barely moisture were as the input variables. the resul...

To investigate the radiation response of hepatoma, SMMC-7721 cells were irradiated with 60 Co γ-rays. Initial chromatid breaks were measured by counting the number of chromatid breaks and isochromatid breaks. A dose-dependent increase in radiation-induced chromatid/isochromatid breaks was observed in G1 and G2 phase respectively. A good relationship was found between cell survival and chromatin...

During meiosis, cohesin complexes mediate sister chromatid cohesion (SCC), synaptonemal complex (SC) assembly and synapsis. Here, using super-resolution microscopy, we imaged sister chromatid axes in mouse meiocytes that have normal or reduced levels of cohesin complexes, assessing the relationship between localization of cohesin complexes, SCC and SC formation. We show that REC8 foci are separ...

background: chromosomal alterations play an important role in carcinogenesis. enhanced chromosomal radiosensitivity is shown for many cancer predisposition conditions including breast cancer. in this study chromosomal radiosensitivity and the frequency of background sister chromnatid exchanges (sce) in lymphocytes of normal individuals and breast cancer patients was compared. materials and meth...

Cytotoxic effects of treating RIF-1, EMT6, and 9L tumor cell lines with c/s-diamminedichloroplatinum(ll) were measured with the sister chromatid exchange assay and compared to results obtained with the colony-forming efficiency assay. The greatest number of sister chromatid exchanges was induced in RIF-1 cells, fewer in EMT6 cells, and the least in 9L cells. Cell survival data obtained with the...