Two simple, sensitive, accurate, rapid, and economical spectrophotometric methods have been developed for the estimation of diacerein in Pharmaceutical dosage forms. Method A is based on the reaction of diacerein with Folin-Ciocalteu reagent, in the presence of 0.5 N sodium hydroxide solution, giving a pink-colored chromogen, which shows maximum absorbance at 512 nm against reagent blank, while...

For evaluation of flavonoid phenols in the low concentration range in white table wines, four colorimetric assays have been compared: (a) reaction with Folin-Ciocalteu reagent before and after precipitation of flavonoids in HCI-methanal (FC); (b) reaction with vanillin (VAN); (c) reaction with 4-(dimethylamino)-cinnamaldehyde (DAC); and (d) a rapid browning test at 60°C in oxidative medium (POM...

Pleurotus ostreatus (MTCC142) was cultivated on three different agro wastes viz: Wheat straw, Soybean straw and Chick pea straw. Total antioxidant, reducing power and total phenolic content effect of the extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and Folin-Ciocalteu method. The aqueous extract of P.ostreatus cultivated on soybean straw exhibited good antioxidant acti...

In this paper, we present a fully integrated and automated lab-on-a-disc for the rapid determination of the total phenolic content (TPC) and antioxidant activity (AA) of beverage samples. The simultaneous determinations of TPC and AA on a spinning disc were achieved by integrating three independent analytical techniques: the Folin-Ciocalteu method that is used to measure TPC, the 2,2-diphenyl-1...

Three different brands of Rose hip (Rosa canina L.) tea were extracted with water, ethanol (EtOH), methanol (MeOH), and aqueous mixtures (50%, v/v) by ultrasound-assisted extraction (UAE) and Soxhlet methods. Total phenolic content was determined according to the Folin-Ciocalteu method. The results were presented by means of the extract yields and total phenolic contents, expressed in gallic ac...

The antioxidant activity, total phenols content and phytochemical constituents of edible portions of four underutilised tropical fruits; Irvingia gabonensis (African mango), Artocarpus altilis (breadfruit), Annona muricata (soursop) and Annona squamosa (sweetsop) were determined. Extracts of fruit samples were screened for selected phytochemicals using standard methods whereas the total phenols...

Effect of various cooking methods on antioxidant content and radical scavenging activity of pumpkin was evaluated. Pumpkin (Cucurbita moschata) was boiled and stir-fried for 2, 4 and 6 minutes respectively. Beta-carotene and lycopene were determined using HPLC and total phenolics measured using Folin-Ciocalteu method. The free radical scavenging activity of the samples was determined using 1, 1...

The variation of antioxidant activity and total phenolic content of some plants commonly used as home remedies were screened on the basis of air drying time of (short term and long term air) the dried plant Ocimum sanctum (leaf), Cucumis sativus (Seed), and ethanolic extracts of Capsicum frutescens (Seed) and Coriandrum sativum (Seed). Antioxidant activities and total phenolic content values we...

In this paper, an active fraction (TA) was obtained from Athyrium multidentatum (Doll.) Ching rhizome by silica gel and Sephadex LH-20 column chromatography. The total phenolic content in TA was determined by Folin-Ciocalteu colorimetric method. Antioxidant activity of TA was evaluated employing various established in vitro systems. As a result, TA showed stong reducing power, metal chelating a...

Two types of monofloral Malaysian honey (Gelam and Nenas) were analyzed to determine their antioxidant activities and total phenolic and flavonoid contents, with and without gamma irradiation. Our results showed that both types of honey can scavenge free radicals and exhibit high antioxidant-reducing power; however, Gelam honey exhibited higher antioxidant activity (p < 0.05) than Nenas honey, ...