INTRODUCTION Established biomarkers for the diagnosis of sepsis are procalcitonin, interleukin 6, and C-reactive protein. Although sepsis evokes changes of coagulation and fibrinolysis, it is unknown whether thromboelastometry can detect these alterations. We investigated whether thromboelastometry variables are suitable as biomarkers for severe sepsis in critically ill adults. METHODS In the...

Atherothrombotic diseases such as myocardial or cerebral infarction are serious consequences of the thrombus formed in blood vessels. Thrombolytic agents are used to dissolve the already formed clots in the blood vessels. However, these drugs have certain limitations which cause serious and sometimes fatal consequences. Herbal preparations have been used since ancient times for the treatment of...

Two Bangladeshi medicinal plants Tridax procumbens and Vernonia cinerea have been investigated for their in vitro thrombolytic, membrane stabilizing and cytotoxic properties. The total phenolic content was also determined and expressed in gallic acid equivalent. Among the two plants, the methanol extract of T. procumbens exhibited highest thrombolytic activity with clot lysis value of 21.15%. S...

WB-3559 A, B, C and D were produced by a bacterium which was classified as a member of the genus Flavobacterium. These compounds were purified by solvent extraction followed by chromatography on silica gel and then isolated by HPLC. The chemical structures were determined on the basis of chemical and spectroscopic evidence as in the succeeding papers. WB-3559 A, B, C and D stimulated rabbit pla...

Four Bangladeshi medicinal plants Withania somnifera, Terminalia arjuna, Moringa olifera and Asparagus racemosus have been investigated for their in vitro thrombolytic and membrane stabilizing. Among the four plants, the methanol extract of W. somnifera exhibited highest thrombolytic activity with clot lysis value of 36.50%. Standard streptokinase and water were used as positive and negative co...

Determination of streptokinase activity is usually accomplished through two assay methods: a) Clot lysis, b) Chromogenic substrate assay. In this study the biological activity of two streptokinase products, namely Streptase®, which is a native product and Heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...

Fibrinogen γ’ is known to influence fibrin clot structure in purified experimental models but little is known regarding its influence on clot structure in plasma. Furthermore, the environmental and biological factors that affect its concentration are poorly described. We analysed fibrinogen γ’, total fibrinogen concentration and fibrin clot structure in 2010 apparently healthy black South Afric...

Introduction: The embolic model of rat middle cerebral artery occlusion is widely used for preclinical testing of neuroprotective drugs. While the time course of the perfusion and diffusion lesion have been described, little data has been collected to describe the time course of clot lysis during tissue plasminogen activator (tPA) administration in this model. To provide another level of charac...

Hematoma lysis with recombinant tissue plasminogen activator (rtPA) has emerged as an alternative therapy for spontaneous intracerebral hemorrhage (ICH). Optimal dose and schedule are still unclear. The aim of this study was to create a reliable in vitro blood clot model for investigation of optimal drug dose and timing. An in vitro clot model was established, using 25 mL and 50 mL of human blo...

In a previous report we showed that plasmin-dependent lysis of a fibrin polymer, produced from purified components, was totally blocked if annexin II heterotetramer (AIIt) was present during fibrin polymer formation. Here, we show that AIIt inhibits fibrin clot lysis by stimulation of plasmin autodegradation, which results in a loss of plasmin activity. Furthermore, the C-terminal lysine residu...