The accuracy and efficiency of Staf-Sistem 18-R (Liofilchem s.r.l., Roseto degli Abruzzi, Teramo, Italy) were compared with those of conventional biochemical methods to identify 523 strains belonging to 16 different human Staphylococcus species. Overall, 491 strains (93.9%) were correctly identified (percentage of identification, > or = 90.0), with 28 (5.4%) requiring supplementary tests for co...

Few studies have been developed to evaluate the differences of bovine mastitis situation between hand and machine milking under the American tropic conditions. Twenty dairy herds were studied, 11 using hand milking (HM) and 9 machine milking (MM), from «San José de las Lajas» municipality, Mayabeque province. Between May 2009 and March 2012, samples from 182 cows (113 MM and 69 HM) were obtaine...

the present study was aimed to identify the frequency of coagulase (coa) and thermonuclease (nuc) genes and staphylococcal enterotoxin a (sea) production among staphylococcus aureus isolated from various sources in shiraz. moreover, the correlation between the sea gene and coagulase and thermonuclease enzymes is also considered. a total of 100 s. aureus were isolated from various sources includ...

A retrospective review of MRSA screening showed that of a total of 8911 patients screened pre-operatively between May 1996 and February 2001, 83 (0.9%) had MRSA isolated from one source or another. During the same period, 115 (13.6%) of 844 positive tissue samples taken during surgery grew Staphylococcus aureus. Of these only 1 (0.01%) was reported to be methicillin-resistant (MRSA). However, a...

The purpose of this study was to determine the minimum number of tests that could be used to differentiate between the coagulase-positive strains of staphylococcus: Staphylococcus aureus, Staphylococcus hyicus, and Staphylococcus intermedius. Eighty coagulase-positive strains of each of the three species were examined. The five tests conducted were growth on modified Baird-Parker agar, growth o...

The ability to rapidly differentiate coagulase-negative staphylococcus (CoNS) from Staphylococcus aureus and to determine methicillin resistance is important as it affects the decision to treat empiric antibiotic selection. The objective of this study was to evaluate CHROMagar S. aureus and CHROMagar MRSA (Becton Dickinson) for rapid identification of Staphylococcus spp. directly from blood cul...

To determine the prevalence of bacterial mastitis in cattle, milk samples positive for California mastitistest (CMT) were cultured during a period of almost 4 years. The bacterial species isolated from 2904 milksamples studied were coagulase negative Staphylococcus sp. in 879 (30.27%) samples, Streptococcusagalactiae in 642 (22.11%), S. dysgalactiae in 332 (11.43%), E. coli in 295 (10.16%), Sta...

"Staphylococcus pettenkoferi" (proposed name) was identified as an unusual agent of osteomyelitis in a diabetic foot infection. The phenotypical tests used failed to give a good identification. Molecular 16S rRNA gene and rpoB sequencing allowed us to correctly identify this new species of coagulase-negative staphylococcus responsible for this chronic infection.

background/objective: neonatal septicemia is one of the major causes of mortality in newborns. the aim of this study is to evaluate the clinical manifestations and laboratory findings in positive blood culture in neonatal septicemia. methods: in this retrospective study, we allocated 100 records positive blood culture of neonates suffering from septicemia. a questionnaire was completed for each...

A total of 288 staphylococcal specimens isolated from different clinical specimens were selected for the evaluation of tests used to detect Staphylococcus aureus. The coagulase (coa) gene PCR was preformed, which confirmed 288 specimens as S. aureus and 51 specimens as coagulase negative staphylococci (CONS). All the specimens were subjected to slide coagulase test, Slidex Staph plus test and t...