Human papillomavirus (HPV) is an etiologic agent of the uterine cervix cancer and several other neoplasias in women globally. E6 protein of HPV type 16 is highly conserved and plays the key role in an inducing cancer via suppressing activity of P. We have used different bioinformatics tools for generation of phylogenetic tree, modeling of RNA secondary structure, gene designing and codon optimi...

In this paper, we analyzed the UL43 gene of duck plague virus (DPV) of codon usage bias. The results may provide a basis for understanding the evolution and pathogenesis of DPV and for selecting appropriate host expression systems to improve the expression of target gene in vitro. In this study, the synonymous codon usage bias of UL43 gene in the 24 herpesviruses have been analyzed and the resu...

Introduction: Helicobacter pylori (H. pylori) infection has remained as a global health problem. Animal studies demonstrated the role of H. pylori oipA gene in the development of gastric cancer. The aim of this study was the cloning and expression of Helicobacter pylori oipA gene in a bicistronic vector harboring mice IL-18 gene. Materials and methods: The target gene encoding oipA was amp...

A novel method for the adaptation of target gene codon usage to most sequenced prokaryotes and selected eukaryotic gene expression hosts was developed to improve heterologous protein production. In contrast to existing tools, JCat (Java Codon Adaptation Tool) does not require the manual definition of highly expressed genes and is, therefore, a very rapid and easy method. Further options of JCat...

The tRNA adaptation index (tAI) is a translation efficiency metric that considers weighted values ( S ij values) for codon–tRNA wobble interaction efficiencies. initial implementation of the tAI had significant flaws. For instance, generated weights were optimized based on gene expression in Saccharomyces cerevisiae , which expected to vary among different species. Consequently, species-specifi...

conclusions in this study, the optimized truncated orf2 protein was expressed in e. coli successfully and the highly purified protein was obtained, which can be a potential vaccine candidate and as an antigen in elisa to diagnose hev infections. results the subcloning was confirmed by pcr, restriction enzyme digestion, and dna sequencing of recombinant plasmid pet30a-orf2.2. the results obtaine...

the alternative use of non-selective herbicides in different years will reduce the possibility of weeds resistance to herbicide by avoiding the crops constant exposure to herbicide selection pressure. the photoperiod-sensitive genic male sterile (pgms) rice with herbicide resistance will be convenient to the mechanization of hybrid seed production and weed control. in order to develop double he...