The accuracy of DNA barcode databases is critical for research and practical applications. Here we apply a frequency matrix to assess sequencing errors in a very large set of avian BARCODEs. Using 11,000 sequences from 2,700 bird species, we show most avian cytochrome c oxidase I (COI) nucleotide and amino acid sequences vary within a narrow range. Except for third codon positions, nearly all (...

Most tropical mammal species are threatened or data-deficient. Data collection is impeded by the traditional monitoring approaches which can be laborious, expensive and struggle to detect cryptic diversity. Monitoring approaches using mammal DNA derived from invertebrates are emerging as cost- and time-effective alternatives. As a step towards development of blowfly-derived DNA as an effective ...

The species of the genus Phaenocarpa Foerster, 1863 (Braconidae: Alysiinae) from South Korea are revised, and is recorded for first time Korea. Three species, artotemporalis sp. nov. , P. brachyura lobata new to Science, two masha Belokobylskij, 1998 fidelis Fischer, 1970, newly recognized in They described illustrated herein with a provision identification key Korean species. In addition, DNA ...

The gypsy moth from Asia (two subspecies) is considered a greater threat to North America than European gypsy moth, because of a broader host range and females being capable of flight. Variation within and among gypsy moths from China (nine locations), one of the native countries of Asian gypsy moth, were compared using DNA barcode sequences (658 bp of mtDNA cytochrome c oxidase subunit 1 [COI]...

DNA barcode (mitochondrial COI) sequences are provided for species identification of aphids from the Korean Peninsula. Most (98%) of the 154 species had distinct COI sequences (average 0.05% intraspecific pairwise divergence) relative to the degree of sequence divergence among species (average value 5.84%). For species in common with other regions, barcodes for Korean samples fell near or withi...

DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a DNA bio-barcode. We collected DNA barcodes, based on COI sequences from 156 species (529 sequences) of fish, insects, and shellfish. We present results on phylogenetic relationships to assess biodiversity the in t...

A second discovery of the echinoid associated shrimp species Diapontonia maranulus Bruce, 1986 is recorded from deep water off Curaçao, Leeward Islands of the Dutch Caribbean. The material is compared with the type description and paratypic material as well as with material of closely related species. A mtDNA COI barcode was obtained from the material. The systematic position of the genus is di...

This study tested the effectiveness of COI barcodes for the discrimination of anuran species from the Amazon basin and other Neotropical regions. Barcodes were determined for a total of 59 species, with a further 58 species being included from GenBank. In most cases, distinguishing species using the barcodes was straightforward. Each species had a distinct COI barcode or codes, with intraspecif...

this study included 81 samples which belonged to 13 speices from myriophyllum genus. both nrdna its1 and its2 and cpdna matk, rbcl and trnh-psba loci were used for diagnose of invasive from native plant. the nrdna its1and its2 data proved highly variable and could differentiate between all but had low pcr amplification success,based on these result they would not recommend as a suitable barcode...

Anthropogenic impacts are an increasing threat to the diversity of fishes, especially in areas around large urban centres, and many effective conservation actions depend on accurate species identification. Considering the utility of DNA barcoding as a global system for species identification and discovery, this study aims to assemble a DNA barcode reference sequence library for marine fishes fr...