The diameter of collagen fibrils in connective tissues, such as tendons and ligaments is known to decrease upon injury or with age, leading to inferior biomechanical properties and poor healing capacity. This study tests the hypotheses that scaffold fiber diameter modulates the response of human tendon fibroblasts, and that diameter-dependent cell responses are analogous to those seen in health...

We have proposed an optical probe that can be used to characterize the orientation of collagen fibers in human dermis. A specific probing ability for collagen results from the use of second-harmonic-generation (SHG) light induced by collagen molecules in the tissue. Based on the concept of collagen SHG light, a reflection-type polarization measurement system (named SHG polarimetry) with a probe...

The percentage of dark. light, and intermediate osteons and their fragments. a\ seen in polarized light, was determined for 53 cross-sections of emhalmed, adult human bone. Dark osteons have collagen fibers predominantly parallel with the long axis of the osteon while in light osteons the fibers are at a sharp angle to the long axis of the osteon. Intermediate osteons

Protein fibers play a crucial role in many disease related phenomena and biological systems. A structural analysis of fibrous proteins often requires labeling approaches or disruptive sample preparation while it lacks chemical specificity. Here we demonstrate that the technique of vibrational sum-frequency scattering (SFS) provides a label-free pathway for the chemical and structural analysis o...

Stromal changes are the key factors for provision of nutrition and growth to any tumor and also they act as a barrier for spread of the tumor. There are limited histochemical studies in the literature on methods to detect, quantify and analyze the collagen in the invasive tumor front (ITF) of oral squamous cell carcinoma (OSCC). Aim: 1) To analyze the nature of collagen (thickness, hue, density...

The objective of the study was to examine the effect of growth-promoting technologies (GP) on Longissimus lumborum steak tenderness, muscle fiber cross-sectional area (CSA), and collagen solubility. Crossbred feedlot heifers ( = 33; initial BW 464 ± 6 kg) were blocked by BW and assigned to 1 of 3 treatments: no GP (CON; = 11); implant, no zilpaterol hydrochloride (IMP; = 11); implant and zilpat...

OBJECTIVE The biomechanical behavior of the sclera determines the level of mechanical insult from intraocular pressure to the axons and tissues of the optic nerve head, as is of interest in glaucoma. In this study, we measure the collagen fiber structure and the strain response, and estimate the material properties of glaucomatous and normal human donor scleras. METHODS Twenty-two posterior s...

Antibodies specific for the alpha 1 (V) chain and native collagen molecules containing the alpha 1 (V) chain have been used in electron immunohistochemical studies of rat kidney to determine the ultrastructural distribution of this class of collagen molecules. In addition, antibodies against type I collagen and whole basement membrane were used as markers for interstitial collagen and authentic...

Quantitative polarized light microscopy (qPLM) is a popular tool for the investigation of birefringent architectures in biological tissues. Collagen, the most abundant protein in mammals, is such a birefringent material. Interpretation of results of qPLM in terms of collagen network architecture and anisotropy is challenging, because different collagen networks may yield equal qPLM results. We ...

This paper describes a noninvasive method for evaluating regional elasticity of atheroma by measuring the minute change in thickness of the arterial wall during one heartbeat. By comparing the pathological findings with the elasticity images, elasticity distributions for lipid and fibrous tissue (mixture of smooth muscle and collagen fiber) were determined in vitro. Furthermore, to characterize...