It is obvious that the factors considered in this paper render data obtained by ultrafiltration open to criticism unless they are checked by other methods and precautions are taken for the elimination of the vitiating effects which have been described. As regards the mechanism of ultrafiltration, the view of a sieve-like action as most experimental evidence indicates, is adequate, if all the fa...

It has been shown experimentally that mixtures of two types of particles, namely, elementary bodies of vaccinia and collodion particles coated with protein, sediment with a single boundary in the analytical centrifuge. Such mixtures have been shown to develop one or two boundaries on electrophoresis in the Tiselius apparatus, depending on the type of coating on the surface of the collodion part...

1. Collodion particles adsorb diphtheria or tetanus or botulinus toxins. These toxins are retained on the particles when washed but are at least in part released in the animal. 2. The adsorbed toxins are neutralized by adsorption of the corresponding antitoxins but are unaffected by other serums. 3. When collodion particles are treated first with tetanus antitoxin, then with diphtheria toxin, t...

Marked differences were noted among 14 antibiotics in their filterability through collodion (nitrated cellulose) membranes. The findings could not be attributed to differences in molecular weight or degree of serum protein binding, although the latter factor imposed an additional restraint on the passage of certain agents. Poorly filtered antibiotics appeared to be absorbed by the collodion. So...

In this note it is intended to complete the proof that the influence of the concentration of electrolytes on the transport of water through a collodion membrane is similar in the case of free and of electrical endosmose. Fig. 1 gives the curves representing the influence of different concentrations of KC1, K~SO4, K3 citrate, and CaC12 upon the initial rate of diffusion of water from pure water ...

1. It is shown that the concentrations of different salts required to precipitate suspensions of gelatin-coated collodion particles in water are practically identical with the concentrations of the same salts required for the "salting out" of gelatin from aqueous solutions. Neither effect shows any relation to the electrical double layers surrounding the particles. 2. It is shown that at the is...

A "paired fibrous structure" was found in "ghost cells" of myeobacteria by Takeya et el. (l) and was supposed to be related to the cell envelope. The present paper will describe further observations on this structure. Cells of Mycobacterium Jucho strain I grown on a thin collodion membrane deposited on 4 per cent glycerol agar were replaced onto the same medium containing isoniazid or streptomy...

In preceding papers *-3 it was shown that electrochemical behavior of collodion membranes (at least in solutions of strong, weakly adsorbable electrolytes) is due to the presence of acidic impurities in the collodion. Highly purified preparations exhibit only low electrochemical activity (as measured by concentration potentials, anomalous osmosis, etc.), and in our opinion, even this low activi...

A procedure for the efficient transfer of cell monolayers, cultured on glass coverslips, to microscopy slides has been developed. This technique involves the coating of the upper surface of an ethanol-fixed cultured cell layer with a film of collodion dissolved in n-amyl acetate. The dry collodion-coated cell layer can then be detached by rehydrating it for 1 h under water or phosphate-buffered...