An N-alkylamide profiling from an ethanolic Anacyclus pyrethrum DC. root extract was performed using a gradient reversed phase high performance liquid chromatography/UV/electrospray-ionization ion-trap mass spectrometry (HPLC/UV/ESI-MS) method on an embedded polar column. MS1 and MS2 fragmentation data were used for identification purposes while UV was used for quantification. Thirteen N-alkyla...

Two commercially available (Abbott Labs.) radioimmunoassays for determination of conjugated cholic acid and sulfoglycolithocholic acid in serum have been modified for bile acid measurements in alcoholic tissue extracts, bile, and urine. The specificity of both radioimmunoassays has been determined with regard to 27 free and conjugated bile acids. After filtration, bile acids can be measured in ...

in pasture-based milk production systems, using available farm resources in order to reduce feeding costs, the composition and functional properties of dairy cows’ milk are of considerable importance to the dairy farmer, manufacturer and consumer. basically, there are three options for altering the composition and/or functional properties of milk: 1) cow nutrition and grazing management, 2) cow...

A new fatty acid metabolite, octadeca-8,11-dienoic acid methylester (1) was isolated together with the known ergosterol (2) from Fistulina hepatica. The edible mushroom Fistulina hepatica is a relatively rarely occurring representative of the wood-rotting basidiomycetes for which no characteristic metabolites have been reported so far. The compounds 1 and 2 were purified by solvent extraction, ...

Conjugated linoleic acid, better known as CLA, is a fatty acid derived from the required nutrient, linoleic acid. The major structural difference between linoleic acid (an 18 carbon, polyunsaturated fatty acid) and CLA, is that linoleic acid has double bonds at carbons 9 and 12 in a cis configuration (methylene interrupted), while CLA has numerous isomers without methylene interrupted conjugate...

Recent studies have indicated that inhibitors of the synthesis of 20-hydroxyeicosatetraenoic acid (20-HETE) may have direct neuroprotective actions since they reduce infarct volume after ischemia reperfusion in the brain without altering blood flow. To explore this possibility, the present study used organotypic hippocampal slice cultures subjected to oxygen-glucose deprivation (OGD) and reoxyg...

background: vaginal atrophy is a common complication in menopause which does not improve with time and, if untreated, can affect the quality of life for women. the aim of this study was to compare the effectiveness of the vaginal cream of hyaluronic acid and conjugated estrogen (premarin) in treatment of vaginal atrophy. methods: this study was a randomized controlled clinical trial on 56 menop...

Conjugated linoleic acid (CLA) is one of the most physiological active fatty acids due to its positive effect on  prevention of diseases such as obesity, hyperlipidemia, atherosclerosis and cancer. The aim of this study was to optimize the production of conjugated linoleic acid (CLA)% in  alkali isomerization of safflower oil including  71% linoleic acid (LA)  using response surface methodology...