Distribution and availability of heavy metals to plants is important when assessing the environmental quality of an area. The objectives of this study conducted in 2002 were: a) determine the levels of the heavy metals cadmium (Cd), copper (Cu), lead (Pb), manganese (Mn), nickel (Ni) and zinc (Zn) in the agricultural soils of the Bursa plain so that the degree of pollution could be ascertained,...

Copper (Cu) is a transition metal and an essential micronutrient for organisms, but also one of the most widespread toxic inorganic contaminants at very high content. The research on Cu isotopes has grown rapidly in the last decade. Hitherto, a large number of studies have been published on the theoretical fractionation mechanisms, experimental data and natural variations of Cu isotopes in vari...

When desialylated ceruloplasmin (ASCPN), labeled with 64Cu and with 3H in its galactose residues, is injected intravenously into a rat, more than half of the radioactivity can be recovered within minutes in ASCPN precipitated immunochemically from liver homogenates. Both radioautographic studies and measurements of hepatic uptake of ASCPN following blockade of the reticuloendothelial system ind...

The reduction potentials (E(0)) of type 1 (T1) or blue copper (BC) sites in proteins and enzymes with identical first coordination spheres around the redox active copper ion can vary by ~400 mV. Here, we use a combination of low-temperature electronic absorption and magnetic circular dichroism, electron paramagnetic resonance, resonance Raman, and S K-edge X-ray absorption spectroscopies to inv...

None of the methods which have been used for isolating Z-(+)lysine from plant extracts and protein hydrolysates is ideal. The oldest method utilizes phosphotungstic acid to precipitate the basic nitrogenous compounds which must be fractionated subsequently (1, 2). In the Kossel-Kutscher silver sulfate-barium hydroxide fractionation (3) the lysine remaining in the final filtrate must be precipit...

We report the supramolecular assembly of artificial metalloenzymes (ArMs), based on Lactococcal multidrug resistance regulator (LmrR) and an exogeneous copper(II)–phenanthroline complex, in cytoplasm E. coli cells. A combination catalysis, cell-fractionation, inhibitor experiments, supplemented with in-cell solid-state NMR spectroscopy, confirmed assembly. The ArM-containing whole cells were ac...

introduction: application of organic fertilizers in agricultural soils with low organic matter content is one of the best ways of nutrientsaddition to these soils. different organic fertilizers have different effects on nutrient availability in soil. moreover study of the distribution of nutrients in the soil allows investigating their mobility and bioavailability. the nutrients availability an...

Crustacean phenoloxidase (PO) and hemocyanin (Hc) are classified as type 3 copper proteins. PO catalyzes the oxidation of mono- di-phenol compounds, which is rate-limiting step melanization, while Hc generally functions a dioxygen-transporting protein in hemolymph arthropods. To date, many studies have shown activity Hc, inspired by their structural similarity. Here, source crustaceans was re-e...

Abstract Dissolved organic matter (DOM) concentrations have been increasing in parts of the northern hemisphere for several decades. This process—brownification—often accompanies iron and aluminum, but metal–DOM interactions these concurrent trends imply are poorly described. Here we used field-flow fractionation with UV ICP-MS detection to measure size distribution colloidal iron, manganese, c...