Anabolic activity, phenotypic alterations, and in particular survival of the chondrocytes are essential for the maintenance of proper articular cartilage and appears to fail during osteoarthritic cartilage degeneration. In this study, we investigated the presence and expression of RhoB in adult human articular cartilage and its regulation in osteoarthritic cartilage as well as in chondrocytes i...

OBJECTIVES To compare the proliferative and chondrogenic potential of fresh and frozen chondrocytes isolated from superficial and deep articular cartilage biopsies. MATERIALS AND METHODOLOGY The study included 12 samples of fresh and frozen healthy human knee articular cartilage. Cell proliferation was tested at 3, 6 and 9 days. Studies of mRNA quantification, protein expression and immunoflu...

Serial studies have been performed on three patients with relapsing polychondritis in an attempt to define a potential immunopathologic role for degradation constituents of cartilage in the causation and/or perpetuation of the inflammation observed. Crude proteoglycan preparations derived by disruptive and differential centrifugation techniques from human costal cartilage, intact chondrocytes g...

In inflammatory diseases such as rheumatoid arthritis, functions of chondrocytes including synthesis of matrix proteins and proteinases are altered through interactions with cells of the infiltrating pannus. One of the major secreted products of mononuclear inflammatory cells is IL-1. In this study we found that recombinant human IL-1f suppressed synthesis of cartilage-specific type II collagen...

articular cartilage, the load-bearing tissue of the joint, has limited repair and regeneration ability. the scarcity of treatment modalities for large chondral defects has motivated researchers to engineer cartilage tissue constructs that can meet the functional demands of this tissue in vivo. cartilage tissue engineering requires 3 components: cells, scaffold, and environment. owning to their ...

OBJECTIVE This study examines the tissue engineering potential of passaged (P3) and primary (P0) articular chondrocytes (ACs) and costal chondrocytes (CCs) from skeletally mature goats for use in the temporomandibular joint (TMJ). DESIGN These four cell types were assembled into scaffoldless tissue engineered constructs and cultured for 4 wks. The constructs were then tested for cell, collage...

31, pp. 273-280, 1996.* Quarto, et al., “Proliferation and Differentiation of Chondrocytes in Defined Culture Medium: Effects of Systemic Factors”, Bone: 17(6) 588, 1995. Quarto, et al., “Modulation of Commitment, Proliferation, and Differentiation of Chondrogenic Cells in Defined Culture Medium”, Endocrinology, 138: 4966-4976, 1997. Yaeger, et al., “Synergistic Action of Transforming Growth Fa...

BACKGROUND The healing potential of articular cartilage in response to injury is poor, because articular cartilage lacks blood vessels or perichondrium. Costal cartilage is covered with a vascularized perichondrium, which is known to have chondrogenic potential. The purpose of this study was to determine whether costal cartilage can heal in response to mechanical injury. METHODS Sixty-five IC...