herein, 1f2, an anti-her2 monoclonal antibody (mab), was covalently coupled to the surface of 5-fluorouracil (5-fu) loaded bovine serum albumin (bsa) nanoparticles. concerning two different crosslinkers for conjugation of 1f2, maleimide-poly (ethylene glycol)-succinimidyl carbonate (mal-peg5000-nhs) was selected due to its higher conjugation efficiency (23±4 %) obtained in comparison to n-succi...

To combat infections on biomedical devices, antimicrobial coatings have attracted considerable attention, including comprising naturally occurring peptides (AMPs). In this study the aim was to explore performance upon extended challenge by bacteria growing in media above samples. The AMPs LL37, Magainin 2, and Parasin 1 were selected basis of well-known membrane disruption activity solution cov...

Liposomes are widely investigated as vaccine delivery systems, but antigen loading efficiency can be low. Moreover, adsorbed may rapidly desorb under physiological conditions. Encapsulation of antigens overcomes the latter problem results in significant loss during preparation and purification liposomes. Here, we propose an alternative attachment method, based on a complementary heterodimeric c...

Herein, 1F2, an anti-HER2 monoclonal antibody (mAb), was covalently coupled to the surface of 5-Fluorouracil (5-FU) loaded bovine serum albumin (BSA) nanoparticles. Concerning two different crosslinkers for conjugation of 1F2, Maleimide-poly (ethylene glycol)-Succinimidyl carbonate (Mal-PEG5000-NHS) was selected due to its higher conjugation efficiency (23±4 %) obtained in comparison to N-succi...

Herein, 1F2, an anti-HER2 monoclonal antibody (mAb), was covalently coupled to the surface of 5-Fluorouracil (5-FU) loaded bovine serum albumin (BSA) nanoparticles. Concerning two different crosslinkers for conjugation of 1F2, Maleimide-poly (ethylene glycol)-Succinimidyl carbonate (Mal-PEG5000-NHS) was selected due to its higher conjugation efficiency (23±4 %) obtained in comparison to N-succi...

Cytochrome c maturation in the periplasms of many bacteria requires the heme chaperone CcmE, which binds heme covalently both in vivo and in vitro via a histidine residue before transferring the heme to apocytochromes c. To investigate the mechanism and specificity of heme attachment to CcmE, we have mutated the conserved histidine 130 of a soluble C-terminally His-tagged version of CcmE (CcmEs...

OBJECTIVE To explore attachment narratives in children diagnosed with reactive attachment disorder (RAD). METHOD We compared attachment narratives, as measured by the Manchester Child Attachment Story Task, in a group of 33 children with a diagnosis of RAD and 37 comparison children. RESULTS The relative risk (RR) for children with RAD having an insecure attachment pattern was 2.4 (1.4-4.2)...

A selective aptasensor was developed using the electrochemical transduction method for the ultrasensitive detection of cocaine. In this method, dendrimer-quantum dot (Den-QD) bioconjugate was utilized as a specific nanocomposite to efficiently fabricate the aptasensor. CdTe QD, which carries highly significant properties, was immobilized on the surface of a glassy carbon electrode (GCE), and po...

Pancreatic and venom phospholipases A2 have complex and distinct oligomerization behaviour. Pancreatic enzymes are monomeric in solution, but their quaternary structure at interfaces is unknown. On the other hand, certain crotalid venom phospholipases A2 are dimeric in solution, and different reports have proposed either the monomer or the dimer as the catalytically functional subunit. In this ...