BACKGROUND The lifesaving treatment endured by cancer patients leads, in many women, to early menopause and subsequent infertility. In clinical situations for which chemotherapy needs to be started, ovarian tissue cryopreservation looks to be a promising option to restore fertility. In 1997, biopsy samples of ovarian cortex were taken from a woman with stage IV Hodgkin's lymphoma and cryopreser...

Background Follicle cryopreservation has been proposed as an alternative fertility preservation option. Follicles can be cryopreserved in intact ovarian tissue pieces or after isolation of individual follicles from the fresh ovarian tissue using enzymatic or mechanical techniques. Researchers have used different cryoprotectants and various techniques to improve cryopreservation. It is plausible...

With the evolution of the treatment of malignant neoplasms, the survival rates of patients undergoing chemo- or radiotherapy are increasing. The continuous development of techniques of assisted human reproduction has led to important strategies in an attempt to maintain reproductive function in patients subjected to treatment of neoplastic diseases, among them cryopreservation of embryos, gamet...

BACKGROUND Cryopreservation of ovarian tissue has been widely accepted as an option for fertility preservation among cancer patients. Some patients are exposed to chemotherapy prior to ovarian tissue cryopreservation. Consequently, assessment of the developmental capacity of human ovarian tissue after chemotherapy is of primary importance. MATERIALS In order to study the impact of previous ch...

Cryopreservation of embryos, oocytes, or ovarian tissues is the main option for female fertility preservation. Oocyte cryopreservation has emerged as especially important: the dramatic increase in the number of infants born from vitrified oocytes indicates that it is becoming one of the most important intervention options. However, oocyte cryopreservation with standard controlled ovarian hypers...

Background: More than a decade work in preservation of male fertility by spermatogonial stem cell (SSC) transplantation in animal models as well as new discoveries about the nature of human SSCs has prompted an investigation into the possibility of an effective clinicalgrade procedure for isolation and cryopreservation of human testicular cells. Materials and Methods: Clinical-grade reagents, v...