Letters 6. Graczyk TK, Cranfield MR, Fayer R. Recovery of waterborne oocysts of Cryptosporidium parvum from water samples by the membrane-filter dissolution method. Parasitol Res 1997;83:121-5. 7. Graczyk TK, Cranfield MR, Fayer R. Evaluation of commercial enzyme immunoassay (EIA) and immunofluorescent antibody (IFA) test kits for detection of Cryptosporidium oocysts of species other than Crypt...

U.S. Environmental Protection Agency method 1623 is widely used to monitor source waters and drinking water supplies for Cryptosporidium oocysts. Matrix spikes, used to determine the effect of the environmental matrix on the method's recovery efficiency for the target organism, require the collection and analysis of two environmental samples, one for analysis of endemic oocysts and the other fo...

Of 471 specimens examined from foxes, raccoons, muskrats, otters, and beavers living in wetlands adjacent to the Chesapeake Bay, 36 were positive for five types of Cryptosporidium, including the C. canis dog and fox genotypes, Cryptosporidium muskrat genotypes I and II, and Cryptosporidium skunk genotype. Thus, fur-bearing mammals in watersheds excreted host-adapted Cryptosporidium oocysts that...

A new species, Cryptosporidium ubiquitum, previously identified as the Cryptosporidium cervine genotype and infrequently as the cervid, W4 or genotype 3 genotype, is described. In published studies this genotype was reported in wild and domesticated ruminants, rodents, carnivores and primates including humans. In the present study oocysts were found in feces from a captive prehensile-tailed por...

Cryptosporidium oocysts were inoculated into fresh dung (∼1.2×10(4) oocysts per gram wet weight) and fed to dung beetles to assess the effect of dung burial by the dung beetle Bubas bison on the distribution of the oocysts in small cores of soil in the laboratory. The experiment consisted of five replicates of each of two treatments; controls (dung but no dung beetles) and the experimental trea...

When determining the recovery efficiency of a procedure for the detection of Cryptosporidium or the removal efficiency of a treatment process, it is necessary to accurately enumerate a 'seed dose'. Conventional techniques for this are highly variable and consequently, can result in misleading data. In this study, a flow cytometric method was developed for the production of suspensions of Crypto...

In order to acquire prevalence and genetic data on Cryptosporidium infections in captive lizards and snakes kept as pets, a survey was conducted on 150 individual reptiles from southern Italy. Fecal samples were preserved in 5% formalin and analyzed using a commercial immunofluorescence assay (IFA) for the detection of Cryptosporidium oocysts and Giardia cysts. IFA revealed the presence of Cryp...

Cryptosporidium has caused the majority of waterborne disease outbreaks in treated recreational water venues in the USA for many years running. This research project evaluated some common US swimming pool filters for removing Cryptosporidium oocysts, 5-µm diameter polystyrene microspheres, and 1-µm diameter polystyrene microspheres. A 946 L hot tub with interchangeable sand, cartridge, and prec...

Quantitative risk assessment for Cryptosporidium oocysts and Giardia cysts was performed to determine the public health significance of non-potable use of tertiary treated reclaimed water. Seven reclaimed water treatment plants in the southwestern United States participated in this study. The average public exposure to oocysts and cysts was estimated, based on concentrations, recovery efficienc...

Inoculation of adult, female BALB/c mice with 2 X 10(5) bleach-treated Cryptosporidium sp. oocysts isolated from calf feces resulted in infection of the uterine mucosa in more than 50% of the animals. Cryptosporidium sp. completed the entire life cycle in the uterus, and infectious oocysts were passed into the vagina. Two methods of application were used to establish intrauterine infection. The...