A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...

Neutrophils contribute to the pathophysiology of hidradenitis suppurativa (HS), a chronic debilitating inflammatory skin disease, yet their exact role remains be fully defined. Granulocyte colony-stimulating factor (G-CSF), major regulator neutrophil development and survival, can blocked by novel, human anti-G-CSF receptor (G-CSFR) monoclonal antibody CSL324. We investigated activation migratio...

Introduction Germinal Matrix-Intraventricular Haemorrhage (GM-IVH) is one of the most common neurological complications in preterm infants, which can lead to accumulation cerebrospinal fluid (CSF) and a major cause severe neurodevelopmental impairment infants. However, pathophysiological mechanisms triggered by GM-IVH are poorly understood. Analyzing CSF that accumulates following IVH may allow...

 Background: Proposing a strategy for sellar reconstruction in endoscopic transsphenoidal transsellar approach for pituitary adenoma. Methods: 240 patients with pituitary adenoma underwent pure endoscopic endonasal transsphenoidal surgery. Intra-operative CSF leaks were classified as grade 0, no observable leak grade 1, CSF dripping through an arachnoid membrane defect of less than 1 ...

in this review the role of cerebrospinal fluid (csf) in mammalian cerebral cortex development has been highlighted. many studies have focused on the potential role of the csf in the developmental process. in particular, the cerebral cortex develops from the germinal epithelium adjacent to the csf. csf contains proteins, growth factors and other neurotrophic factors which are important for neura...

A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...

Background: Human granulocyte colony-stimulating factor (hG-CSF) can serve as valuable biopharmaceutical for research and treatment of the human blood cancer. Transplastomic plants have been emerged as a new and high potential candidate for production of recombinant biopharmaceutical proteins in comparison with transgenic plants due to extremely high level expression, biosafety and many other a...