background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...

Capsicum chinense is a small aromatic pepper native to the Amazon and widely used in Caribbean Basin. Most types of C. are highly pungent, but landraces Puerto Rico generally have little or no pungency. This “sweet chili pepper” (or ají dulce, as it known on Island) an integral part local cuisine. Ten sweet lines derived from non-pungent were evaluated Lajas, Juana Díaz Corozal, Rico, 2009. The...

cucumber mosaic virus (cmv) belongs to the genus cucumovirus in the family bromoviridae. althaea officinalis, commonly known as hollyhocks in the family malvaceae, is an annual plant whose fruit, flower and root are used as medicinal products. in this study, five samples of hollyhocks leaves with viral symptoms were collected and cmv was detected using das-elisa from the samples. ta-alth isolat...

Cucumber mosaic virus (CMV), the type member of the genus Cucumovirus (family Bromoviridae), is transmitted by aphids in a nonpersistent manner. Mutagenesis experiments identified the betaH-betaI loop of the capsid subunit as a potential key motif responsible for interactions with the insect vector. To further examine the functional characteristics of this motif, we generated monoclonal antibod...

Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...