The study recorded for the first time in Egypt presence of tomato spotted wilt virus (TSWV) on cucurbits, which was confirmed using several serological tests, like double antibody sandwich ELISA (DAS-ELISA); Dot-blot assay and Immuno capture polymerase chain reaction (IC-PCR).  By Dot blot immunoassay test against TSWV antisera, ability thrips species Thrips tabaci (Lind), palmi (Karny), Frankl...

A highly sensitive one-step multiplex RT-PCR (OMRT-PCR) system with an internal plant gene control was developed to simultaneously detect single and mixed RNA DNA viral pathogens affecting watermelon squash in the Southeastern United States. Current methods rely on a singleplex detection for several viruses which is time-consuming large-scale field monitoring. The gene-specific primers one viru...

Ampelomyces quisqualis CPA-9 was reported as a promising biocontrol fungus against powdery mildew on cucurbits, although its formulation had not been developed. The present work aimed to develop fluidised-bed spray-dried of that included film-forming compounds, which could improve the behaviour microorganism under practical conditions. Film forming efficacy, shelf life, physical stability funct...

Papaya ringspot, caused by papaya ringspot virus, is the most important disease of the crop in Florida, severely limiting the potential for commercial production. Sometimes this virus infects cucurbits. Earliest symptoms in papaya appear as yellowing and vein-clearing of younger leaves. A prominent yellow mottling of the leaves follows. One or more lobes of infected leaves may become severely d...

Serological assays were used to estimate the proportion of Acalymma vittata (F.) that harbored Erwinia tracheiphila (E. F. Smith) Holland, the causal agent of bacterial wilt in cucurbits. Theseproportionswere related to theproportionofA. vittata that transmitteddisease in singlebeetle caged bioassays. The serological assays classiÞed beetles as harboring the bacteria when the titer was above '1...