introduction: the suspected water samples contaminated with legionella were enriched with acanthamoebae. small numbers of bacteria (not detectable by culture) can be multiplied in amoebae up to culturable level in co-culture condition. the conventional culture method for legionnaires' disease bacterium always suffers from low sensitivity. this co-culture method can significantly improve culture...

  A total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in Mashhad. The presence of Salmonella was assessed by conventional culture method and confirmed by using poly O and poly H antiserum in serotyping. PCR amplification of invA gene as a specific method for detection of Salmonella w...

mycoplasma synoviae (m. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. this study was designed to detect m. synoviae through culture isolation and polymerase chain reaction (pcr) assay to demonstrated the involvement of m. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...

conclusions performing only culture method led to missed false negative carriers. therefore, it is recommended that both the pcr assay and conventional culture method be routinely performed to detect gbs in pregnant women accurately. pcr diagnosis demonstrated a shorter turnaround time when compared with the time consuming culture method. results the prevalence of gbs in the 203 collected sampl...