B-acute lymphoblastic leukemia (B-ALL) is the frequent pediatric malignity. Chemotherapy is the most practical approaches to deal with such malignancies. Microtubule-targeted agents are one of the most strategic drugs which formerly use in chemotherapy.Although,colchicine-binding anti-tubulin agents exhibited promising effects in clinical trials, their exact mechanism of action is not fully und...

The reductively dimethylated derivatives of horse and yeast iso1-ferricytochromes c have been prepared and characterized for use as NMR probes of the complexes formed by cytochrome c with bovine liver cytochrome b & and yeast cytochrome c peroxidase. The electrostatic properties and structures of the derivatized cytochromes are not significantly perturbed by the modifications ; neither are the ...

The cytochrome b-c1 complex has been “solubilized” and purified to a stage containing 6.5 nmoles of cytochrome b per mg of protein. Seven bands are resolved on a polyacrylamide gel electrophoretic column in sodium dodecyl sulfateP-mercaptoethanol medium. Five of these seven bands have been identified as cytochromes b and cl and a non-heme iron protein. The remaining two bands might be associate...

In the course of studies by Keilin and Hartree (1) on succinic oxidase preparation of heart muscle particles, it was observed that the kinetics of reduction of cytochrome b were not in accord with some rather fundamental criteria that must be met for an electron-transferring component in the respiratory chain (2). On the other hand, studies of the kinetics of cytochrome b in intact yeast cells ...

Energy transduction in the cytochrome bc(1) complex is achieved by catalyzing opposite oxido-reduction reactions at two different quinone binding sites. We have determined the pre-steady state kinetics of cytochrome b and c(1) reduction at varying quinol/quinone ratios in the isolated yeast bc(1) complex to investigate the mechanisms that minimize inhibition of quinol oxidation at center P by r...

Biochemical analyses of Rubrivivax gelatinosus membranes have revealed that the cytochrome bc(1) complex is highly resistant to classical inhibitors including myxothiazol, stigmatellin, and antimycin. This is the first report of a strain exhibiting resistance to inhibitors of both catalytic Q(0) and Q(i) sites. Because the resistance to cytochrome bc(1) inhibitors is primarily related to the cy...

In the course of studies by Keilin and Hartree (1) on succinic oxidase preparation of heart muscle particles, it was observed that the kinetics of reduction of cytochrome b were not in accord with some rather fundamental criteria that must be met for an electron-transferring component in the respiratory chain (2). On the other hand, studies of the kinetics of cytochrome b in intact yeast cells ...

The electron-transfer reactions of cellobiose oxidase (CBO) have been investigated by conventional and by rapid-scan stopped-flow spectroscopy at pH 6.0. Analysis of the absorbance/time/wavelength matrix by Singular Value Decomposition (SVD) confirms earlier studies showing that cellobiose rapidly reduces the flavin group (7.7 s-1; cellobiose, 100 microM) which in turn slowly (0.2 s-1) reduces ...

The cytochrome b-c1 complex has been “solubilized” and purified to a stage containing 6.5 nmoles of cytochrome b per mg of protein. Seven bands are resolved on a polyacrylamide gel electrophoretic column in sodium dodecyl sulfateP-mercaptoethanol medium. Five of these seven bands have been identified as cytochromes b and cl and a non-heme iron protein. The remaining two bands might be associate...