Immunotherapy has raised the attention of many scientists because it hold promise to be an attractive therapeutic strategy to treat a number of disorders. In this study, the immunomodulatory effects of low titers of Newcastle disease virus (NDV) AF2240 on human peripheral blood mononuclear cells (PBMC) were analyzed. We evaluated cytokine secretion and PBMC activation by cell proliferation assa...

BACKGROUND/AIM Interleukin-12 (IL-12) and interferon gamma (IFN-γ) are key cytokines in immunemediated equine melanoma therapy. Currently, a method for accurate simultaneous quantification of these equine cytokines is lacking. Therefore, we sought to establish an assay that allows for accurate and simultaneous quantification of equine IL-12 (eIL-12) and IFN-γ (eIFN-γ). MATERIALS AND METHODS S...

In the present communication, we report on exploitation of a Bloch surface wave-enhanced fluorescence scheme for detection Interleukin (IL)-10 in protein-rich buffer mimicking biological sample. IL-10 is cytokine known its potent anti-inflammatory and immunosuppressive effects. It considered valuable biomarker prognostic prediction both solid tumors hematological malignancies, recently, disting...

We previously reported a screening method for caloric restriction mimetics (CRM), group of plant-derived compounds capable inducing good health and longevity. In the present study, we explored possibility using this to screen CRM drugs drug repositioning. The method, T-cell activation-inhibitory assay, is based on inductive logic. Most such as resveratrol have been suppress activation anti-infl...

reaction is 2 ␮L). If probes are labeled with two different dyes (LCRed640 and LCRed705), two independent genes can be quantified in a single reaction, doubling the number of targets. Other methods, such as intracellu-lar cytokine staining, require at least 10 6 cells for the quantification of only one cytokine. Thus the proposed method enables determination of different subpopula-tions of cell...

Introduction: Interleukin-2 (IL-2) as a T helper type 1 (Th1) cytokine has an important role Background and Objective: Interleukin-2 (IL-2) as a T helper type 1 (Th1) cytokine has an important role in activation, growth and differentiation of several immune cells. Moreover IL-2 is known as a pro-inflammatory and anti-tumoral cytokine. In addition deysregulation of IL-2 in some diseases such as ...

Abstract Many patients who have undergone allogeneic hematopoietic stem cell transplantation (aHSCT) as part of cancer therapy achieve remission. However, nearly 50% these develop Graft versus Host Disease (GVHD), a chronic autoimmune disease that can damage eyes. Quantifying specific cytokine changes in tears may reveal biomarkers and future treatment targets for ocular GVHD (oGVHD). Compared ...

A Luminex (Luminex Corp., Austin, TX) multiplex swine cytokine assay was developed to measure 8 cytokines simultaneously in pig serum for use in assessment of vaccine candidates. The fluorescent microsphere immunoassay (FMIA) was tested on archived sera in a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine/challenge study. This FMIA simultaneously detects innate (IL-1 beta, I...