OBJECTIVE The present study aimed to simultaneously evaluate the association between expression of three potential factors [post-acrosomal sheath WW domain-binding protein (PAWP), phospholipase Cζ (PLCζ), and truncated form of the kit receptor (TR-KIT)] as candidates of oocyte activation with fertilization rate and early embryonic development. MATERIALS AND METHODS In this experimental study,...

introduction: sperm viability test is important in diagnosis and treatment of male infertility. the common test is e&n; and host which they are based on membrane permeability and integrity. the aim of this study was to evaluate mtt assay as a sperm viability test based on mitochondrial activity. materials and methods: washed sperm samples were co-incubated with mtt in different media and differ...

background: c-reactive protein (crp) can be increased after hormonal stimulations. the changes of crp might affect the success of in-vitro fertilization (ivf). objective: the aim of this study was to determine the possible relationship between the serum crp level and outcome of controlled ovarian stimulation, and pregnancy rate in patients undergoing ivf or intra cytoplasmic sperm injection (ic...

in human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. the sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. the objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...

background: intracytoplasmic sperm injection (icsi) is an advanced technique employed in assisted reproductive clinics for treatment of infertile couples. the reproductive endocrinologists try their level best to identify factors that enhance success rate after icsi. objective: to compare estradiol progesterone ratio on ovulation induction day amongst pregnancy outcome groups following icsi. ma...

Assisted oocyte activation (AOA) is the technique that simulates the effect of sperm penetration into an oocyte inducing oocyte activation. This technique is used in combination with intracytoplasmic sperm injection (ICSI) to improve the outcome of the fertilization process. It is mostly beneficial and recommended in those cases, when classic ICSI fails. This accounts approximately up to 5 % of...

BACKGROUND Irradiation is one of the major causes of induced sperm DNA damage. Various studies suggested a relation between sperm DNA damage and fertilization rate after intra-cytoplasmic sperm injection (ICSI). OBJECTIVE In this study, fertilization rate and premature chromosome condensation (PCC) formation after ICSI of hamster oocytes with irradiated sperms from normal and oligosperm indiv...