Cytotoxicity of cadmium sulphide, oxide and chloride was tested using A549 and CHO-9 cells. Metabolic activity of cells (MTT test) and cell membrane permeability (NRU test) were used as cytotoxicity endpoints. The results revealed unexpectedly low toxicity of cadmium sulphide as compared to chloride and oxide. This preliminary report does not provide any explanation for this effect, but the res...

This paper proposes a new cytotoxicity assay in a microfluidic device with microwells and a distributive microfluidic channel network for the formation of cancer cell spheroids. The assay can generate rapid and uniform cell clusters in microwells and test in situ cytotoxicity of anticancer drugs including sequential drug treatments, long term culture of spheroids and cell viability assays. Inle...

The majority of mycotoxins produced by Aspergillus fungi are immunosuppressive agents, and their cytotoxicity may impair defense mechanisms in humans. The objective of the study was evaluation of the cytotoxicity of fungi isolated from an environment where inpatients with impaired immunity were present. The materials comprised 57 fungal strains: Aspergillus fumigatus, Aspergillus niger: Aspergi...

lactic acid bacteria which are  used  as  probiotics  have  ability to  modulate  immune responses and modify immune mechanisms. it has also been indicated that some strains of this family can affect the immune responses against solid tumors. in the present work, we proposed to study the effects of oral administration of l.cacesi ssp casei on the nk cells cytotoxicity and also production of cyt...

large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested before their validation as fi lling materials. the aim of this study was to evaluate the cytotoxic effect of a new copaiba oil-based root canal sealer (biosealer [bs]) on osteoblast-like osteo-1 cells. materials and methods: the experimental groups were formed according to the culture medium cond...

This article describes the preparation and characterization of anticancer drug-loaded poly(lactide-coglycolide) (PLGA) microparticles. PLGA microparticles loaded with doxorubicin HCl (DOX) were prepared via o/w emulsion solvent evaporation. The release characteristics, encapsulation efficiency, size, and morphology of the PLGA microparticles were also determined. A cytotoxicity test was perform...

BACKGROUND Vanda tessellata (Orchidaceae) has been used in different sorts of ailments such as inflammations, rheumatism, dysentery, bronchitis, dyspepsia and fever in folk medicine. In this study we evaluated the antinociceptive and cytotoxic effect of methanol and aqueous extracts of V. tessellata leaf. METHODS Oral administration of V. tessellata aqueous (VTA) and methanol (VTM) leaf extra...

OBJECTIVE The purpose of this in vitro study was to compare the cytotoxicity of 0.5-4.5 origanum extract solution (OES), 2% chlorhexidine gluconate (CHX) and 5.25% sodium hypochlorite (NaOCl) with WST-1 test on human periodontal ligament (hPDL) fibroblasts. MATERIALS AND METHODS About 0.5-4.5% OES, 2% CHX and 5.25% NaOCl solutions cytotoxicity was evaluated with cell culture test using PDL fi...

introduction: this in vitro study assessed and compared the cytotoxicity of mineral trioxide aggregate (mta), calcium-enriched mixture (cem) cement, biodentine (bd) and octacalcium phosphate (ocp) on stem cells of the human apical papilla (scap). methods and materials: scaps were isolated from two semi-impacted third molars. the cells were cultured in wells of an insert 24-well plate and were t...

It was demonstrated that an extract from the pharmacopoeial raw material bilberry fruit (Fructus Myrtilli) co-administered with selected antibiotics: cefuroxime, cefoperazone and doxycycline, positively affects the survival of mouse thymocytes in cultures with hydrocortisone (cytotoxicity test), increases the count of splenocytes forming spontaneous rosettes (E-rosette test) and increases the a...