Isotopic labeling of RNA with 13C and 15N has become a routine procedure in structural studies by NMR spectroscopy. The methodology in this paper describes the random fractional deuteration of RNA using the obligate methylotropic bacterium, Methylophilus methylotrophus. This bacterium was grown using a non-deuterated carbon source in 52:48 D20/H20 and we have shown that all protons in the ribon...

In the legend for Figure 4, there is a misspelling. The correct spelling is: 50 mm. The authors have provided a corrected version here. Figure 5 is incorrect. Figure panel 5H was an inadvertent duplication of the figure panel 5G. Figures 5G and H show ELISA quantifications of extra(EC) and intracellular (IC) S1P levels in 3T3 cells (G) and cerebellar granule neurons (H) before and after 30 and ...

OH, N2, H2, and trans la t Laboratory experiments in which recombined CO, C02, D20, and 02 molecules desorb from surfaces in excited internal ional states are briefly reviewed. Unequilibrated distributions predominate from the principally catalytic-metal substrates so far investigated. and in some cases less than, wall-thermal; the velocity distributions generally vary with emission angle, with...

This study estimates the multi-component efficiency of banks under financial holding companies, including profitability and marketability efficiencies, by utilizing a RAM (Range-adjusted measure) variation model. The analysis process uses the three-dimensional BCG (Boston Consulting Group) matrix created by this study. We find that although semi-publicly-owned banks under financial holding comp...

We study how diversity evolves at a firm with entry-level and upper-level employees who vary in ability and "type" (gender or ethnicity). The ability of entry-level employees is increased by mentoring. An employee receives more mentoring when more upper-level employees have the same type. Optimal promotions are biased by type, and this bias may favor either the minority or the majority. We char...

Surface Enhanced Raman Scattering, Hydrogen Exchange, Nucleic Acids The enhanced Raman effect from 5'-AMP adsorbed on the roughened silver surface is used to characterize hydrogen-deuterium exchange. SERS spectra of 5'-AMP present in HzO and D20 solutions at rather low concentrations were recorded in the spectral range of 2000 to 3500 cm-1. Surface enhanced Raman bands in this spectral range ar...

A multiproduct cost function is estimated for English higher education institutions using a panel of data from recent years. The panel approach allows estimation by means of a random parameter stochastic frontier model which provides considerable new insights in that it allows the impact on costs of inter-institutional differences in the cost function itself to be distinguished from inter-insti...

A gas-pressure cell, based on a leuco-sapphire single-crystal, serving as pressure vessel and sample holder, is presented for real time in situ studies of solid-gas hydrogenation reactions. stainless steel corpus, coated with neutron absorbing varnish, allows alignment the single-crystal holder minimizing contributions to diffraction pattern. Openings corpus enable scattering well contactless t...

Background: Human Adenovirus species D (HAdV-D) was common human viral pathogen especially in eye infection, consists of several types of which HAdV-D8, -D19 and –D37 were common in eye infection. This study includes detection of HAdV-D types implicated in conjunctivitis based on L2 (Penton protein) gene similarity. Methods: Conjunctival swabs were collected from Keratoconjunctivitis patient...

SV-40 virus-transfromed human diploid fibroblasts (2RA) were grown in a monolayer on plastic Petri dishes in an aqueous medium deuterated to different concentrations of deuterium oxide: 10, 20, 30, up to 60%. The cells must be acclimatized to concentrations higher than 20% D2O by stepping them from a lower initial concentration during their exponential growth. The increase of cell doubling time...