We describe the synthesis and fluorescence properties of a Fura-2FF-based fluorescent Ca(2+) indicator that can be covalently linked to SNAP-tag fusion proteins and retains its Ca(2+) sensing ability after coupling to protein.

In the educational context it is examined the teacher-stress association. The teacher’s stress is related with variables of academic environment, of out of the school and of the teacher. The impact of that association can be see in the teacher’s health, in his achievement and in the quality of the teaching-learning process. With the objective to present one view of how issue is being considered...

We demonstrate that an oft-used indirect attitude assessment technique—the attitude activation paradigm—accurately assesses attitudes only when participants attend to the prime stimuli during the attitude activation task. Attitude activation attitudes toward obviously valenced words (e.g., torture, liberty) were more sensitive to attitude valence and extremity when participants were required to...

Etiopathogenesis of the laryngopharyngeal reflux has not been sufficiently clarified. It is believed that damage to the lining of laryngopharynx in laryngopharyngeal disease occurs in the use of acid and pepsin. The diagnosis of reflux acidic 24-hour Dual probe pH-metry is considered the gold standard. However, since the laryngopharyngeal reflux is often non-acidic new diagnostic methods are be...

Warnings can help prevent damage and harm if they are issued timely and provide information helping responders and population to adequately prepare for the disaster to come. Today, there are many indicator and sensor systems that are designed to reduce disaster risks, or issue early warnings. In this paper we analyze the perception that people have from security management systems and we propos...

UV-induced DNA damage gives rise to mutations and skin cancer. We have previously reported that treatment of skin cells in vitro with thymidine dinucleotide (pTT) activates p53 and increases the ability of cells to repair subsequent UV-induced DNA damage by enhancing endogenous DNA repair capacity. Here we show that topical pTT pretreatment enhances the rate of DNA photoproduct removal, decreas...