A mutant strain of Neurospora crassa blocked in each of the initial steps of tryptophan, tyrosine, and phenylalanine biosynthesis was previously shown to accumulate and secrete prephenate and L-arogenate (Jensen, R.A., Zamir, L.O., St. Pierre, M., Patel, N., and Pierson, D.L. (1977) J. Bacteriol. 132, 896-903). We now report the co-accumulation of yet another compound which was identified (Zami...

BACKGROUND This study was performed to biochemically assess and quantify the previously observed ultrastructural alterations in the collagen matrix of stunned myocardium. METHODS AND RESULTS The stunned myocardium was produced in 13 mongrel dogs by a series of 12 coronary artery occlusions of 5 minutes followed by 10-minute reperfusion periods, with a final reperfusion period of 90 minutes. R...

The enzyme cystathionase from rat liver is inactivated by incubation with 5,5’-dithiobis(Z-nitrobenzoic acid) at pH 7.4. The reaction of four -SH groups per mole of enzyme brings about 95% loss of the homoserine deaminase activity. Kinetically, the reactive --SH groups can be classified into two classes. The substrate L-homoserine or the competitive inhibitor L-alanine has no effect on the rate...

Dansyl-anthracene dyads 1 and 2 in CH(3)CN-H(2)O (7:3) selectively recognize Cu(2+) ions amongst alkali, alkaline earth and other heavy metal ions using both absorbance and fluorescence spectroscopy. In absorbance, the addition of Cu(2+) to the solution of dyads 1 or 2 results in appearance of broad absorption band from 200 nm to 725 nm for dyad 1 and from 200 nm to 520 nm for dyad 2. This is a...

Melittin is a cationic hemolytic peptide isolated from the European honey bee, Apis mellifera. In spite of a number of studies, there is no consensus regarding the orientation of melittin in membranes. In this study, we used a melittin analogue that is covalently labeled at its amino terminal (Gly-1) with the environment-sensitive 1-dimethylamino-5-sulfonylnaphthalene (dansyl) group to obtain i...

For the first time, retinol has been associated with Vitreoscilla filiformis extract (a probiotic fraction regenerative properties). The efficacy of this new combination was assessed in vitro and confirmed clinical trials. Normal human keratinocytes were treated or not Retinol (0.1 μM), (1.4 x 10-4%) their for 24 hours. Gene expression then evaluated by RT-qPCR. In addition, trials performed to...

Fluorescent beta-galactosides (1-(N-dansyl)amino-beta-D-galactopyranoside (DG0), 2'-(N-dansyl)aminoethyl-beta-D-thiogalactopyranoside (DG2), 2'-(N-dansyl)aminoethyl-beta-D-galactopyranoside (oxy-DG2), and 6'-(N-dansyl)aminohexyl-beta-D-thiogalactopyranoside (DG6)) competitively inhibit lactose transport by membrane vesicles from Escherichia coli ML 309-225, but are not actively transported. An ...

The biogenic amine (BA) content in wines is dependent on the fermentation processes and other oenological practices, as well grape quality. These compounds can participate different cellular functions humans; however, intake of high amounts provoke some toxicological effects. For that reason, controlling evolution amines wine production extreme importance. This work aims to assess occurrence sp...