Insulator-based (electrodeless) dielectrophoresis (iDEP) is an innovative approach in which the nonuniform electric field needed to drive DEP is produced by insulators, avoiding problems associated with the use of electrodes. Live and dead Escherichia coli were concentrated and selectively released by applying stepped DC voltages across a microchannel containing an array of insulating posts etc...

This paper describes a computational model of spatial learning and localization in rodents. The model is based on the suggestion (based on a large body of experimental data) that rodents learn metric spatial representations of their environments by associating sensory inputs with dead-reckoning based position estimates in the hippocampal place cells. Both these sources of information have some ...

An image analysis program and protocol for the identification and enumeration of live versus dead cells of the yeast-like fungus Aureobasidium pullulans was developed for both populations on microscope slides and leaf surfaces. Live cells took up CellTracker Blue, while nonviable cells stained with DEAD Red. Image analysis macro programs running under Optimas software were used to acquire image...

Evaluation of the acrosome reaction can shed light on the fertilising competence of spermatozoa. To eliminate false-positive results when evaluating the acrosome status of human sperm cells, two viability probes propidium iodide (PI) and 7-amino-actinomycin D (7-AAD) were compared for their ability to stain nonviable cells post-fixation and permeabilisation. Both the mean fluorescence and % dea...

A good audiologic diagnosis is increasingly more important in the practice of audiology, in order to understand patients' needs for selection and fitting of hearing aid devices. Aim: Show recent literature that mention the concept of cochlear dead regions, diagnostic strategies and its relevance in the process of selection and fitting of hearing aids. Methods: to carry out a bibliographical sur...

Background: Human peripheral blood NK cells constitutively express CD56 and CD16 antigens. Peripheral blood NK cells seem to be strongly related with decidual NK cells, and may reflect the decidual NK cell functional status. There are varied reports concerning the relationship between NK cell cytotoxicity in women with recurrent spontaneous abortion. Objective: To study NK activity in women wit...

background: spermatogonial stem cells (sscs), a subset of undifferentiated type a spermatogonia, are the foundation of complex process of spermatogenesis and could be propagated in vitro culture conditions for long time for germ cell transplantation and fertility preservation. objective: the aim of this study was in vitro propagation of human spermatogonial stem cells (sscs) and improvement of ...

UNLABELLED A good audiologic diagnosis is increasingly more important in the practice of audiology, in order to understand patients needs for selection and fitting of hearing aid devices. AIM Show recent literature that mention the concept of cochlear dead regions, diagnostic strategies and its relevance in the process of selection and fitting of hearing aids. METHODS to carry out a bibliog...

Understanding the behaviours of swimming microorganisms in various environments is important for understanding cell distribution and growth in nature and industry. However, cell behaviour in complex geometries is largely unknown. In this study, we used Tetrahymena thermophila as a model microorganism and experimentally investigated cell behaviour between two flat plates with a small angle. In t...