The physicochemical characterization of the textile industry effluent collected from Oswal Textile Industries, Ludhiana (Punjab.) India has been carried out and the results showed that the temperature (40°C), pH (8.00), Biological Oxygen Demand (260 mgl –1 ), Chemical Oxygen Demand (790 mgl –1 ), Total Suspended Solids (2000 mgl –1 ), Total Dissolved Solids (7000 mgl –1 and colour over the pres...

BACKGROUND AND OBJECTIVES Synthetic dyes are recalcitrant to degradation and toxic to different organisms. Decolorization of textile wastewaters is one of the major concerns since last decades. Physical-chemical treatments are very expensive and frequently producing large amounts of toxic wastes. Biological treatments can be more convenient. In the present study, an attempt has been made for de...

To achieve effective decolorization of reactive dyes, laccase immobilization was investigated. Laccase 0.2% (m/V) (Denilite IIS) was trapped in beads of alginate/gelatin blent with polyethylene glycol (PEG), and then the supporters were activated by cross-linking with glutaraldehyde. The results of repeated batch decolorization showed that gelatin and appropriate concentration of glutaraldehyde...

The increasing use of synthetic dyes is alarming and their discharge as textile waste may cause substantial ecological damage. Biological decolorization of dye using microorganisms is an environmentally friendly and costcompetitive alternative to chemical methods. This study involves the application of laccase obtained from Pleurotus ostreatus, a common fungus, in decolorization and degradation...

An electrochemical treatment (EC) was applied at different intensities to degrade the chromophoric groups of dyes C.I. Reactive Black 5 (RB5) and C.I. Reactive Blue 7 (Rb7) until uncolored species were obtained. Decolorization rate constants of the azo dye RB5 were higher than the phtalocyanine Rb7 ones. In addition, the EC treatment was more efficient at higher intensities, but these condition...

Results Materials and Methods Four commercial dyes were used — Remazol® Black BA, Levafix® Blue Ca, Levafix® Yellow CA and Levafix® Red CA (Dystar). A standard anthraquinone dye, Remazol Brilliant Blue R (RBBR, Sigma) was also used. Dye stock solutions were added to solid or liquid decolorization media, up to 200 mg.L-1 final concentration. Rose Bengal Agar and Potato Dextrose Agar (both from D...

BACKGROUND Laccases have good potential as bioremediating agents and can be used continuously in the immobilized form like many other enzymes. METHODS In the present study, laccase from Cyathus bulleri was immobilized by entrapment in Poly Vinyl Alcohol (PVA) beads cross-linked with either nitrate or boric acid. Immobilized laccase was used for dye decolorization in both batch and continuous ...

In the present study, a comparison of central composite design (CCD) and Taguchi method was established for Fenton oxidation. [Dye]ini, Dye:Fe(+2), H2O2:Fe(+2), and pH were identified control variables while COD and decolorization efficiency were selected responses. L 9 orthogonal array and face-centered CCD were used for the experimental design. Maximum 99% decolorization and 80% COD removal e...

The detail understanding of physiological/biochemical characteristics of individual laccase isoenzymes in fungi is necessary for fundamental and application purposes, but our knowledge is still limited for most of fungi due to difficult to express laccases heterologously. In this study, two novel laccase genes, named lac3 and lac4, encoding proteins of 547 and 532-amino acids preceded by 28 and...

Extracellular laccase was produced by Trametes versicolor IBL-04 using corn cobs as a substrate under pre-optimized culture conditions. A 64kDa laccase enzyme was purified and immobilized on calcium alginate beads using glutaraldehyde as a cross-linking reagent. Maximum enzyme immobilization efficiency (89%) was observed with 2-mm calcium-alginate beads that were developed using 4% (w/v) sodium...