A denaturing gradient gel electrophoresis (DGGE) assay was developed to rapidly discriminate species of avian Eimeria. Amplification by PCR of the small subunit ribosomal RNA gene (approximately 1,600 nucleotides) with Eimeria genus-specific primers followed by cloning and sequencing allowed us to carry out phylogenetic analyses and identify clone sequences to species level in most cases. Clone...

Ghee is widely produced from a traditional fermented butter-like product named mashita in western Uganda. However, no detailed studies have been done to identify the microorganisms involved in mashita fermentation. The aim of this study was to identify the microorganisms present at the end of mashita ripening using culture-dependent and culture-independent techniques. The most commonly identifi...

agricultural sustainability is closely related with the efficient use of natural resources, which are primarily transformed by the action of microorganisms. soil microorganisms are usually used as early indicators of soil quality since they rapidly respond to changes in soil management. a field experiment was carried out aiming to evaluate the effect of siratro (macroptylium atropurpureum), bah...

Recirculation technology has been emerging in the marine aquaculture industry. The microbiome developed recirculation systems (RASs) is an important factor for optimal operation of these and fish welfare. In this study, microbial community dynamics water column biofilms a RAS with Mediterranean species gilthead sea bream bass were investigated, while physicochemical conditions also monitored. M...

Mezcal is the second most important alcoholic beverage produced in Mexico, and microorganisms present during fermentation process are primarily responsible for its organoleptic characteristics. Among these, bacterial populations have been less studied, mainly due to difficulty of recovering them. The aim was apply a culture-independent molecular technique detect involved spontaneous mezcal Dura...

Using 16S rRNA gene-based approaches, we analyzed the responses of ileal and colonic bacterial communities of weaning piglets to dietary addition of four fermentable carbohydrates (inulin, lactulose, wheat starch, and sugar beet pulp). An enriched diet and a control diet lacking these fermentable carbohydrates were fed to piglets for 4 days (n = 48), and 10 days (n = 48), and the lumen-associat...

The diversity of different bacterial groups of activated sludge samples that received wastewater from four different types of industry was investigated by a nested PCR-DGGE (denaturing gradient gel electrophoresis) approach. Specific 16S rRNA primers were chosen for large bacterial groups (Bacteria and α-Proteobacteria in particular), which dominate activated sludge communities, as well as for ...

Bacterioplankton community structures under contrasting subtropical marine environments (Hong Kong waters) were analyzed using 16S rRNA gene denaturing gradient gel electrophoresis (DGGE) and subsequent sequencing of predominant bands for samples collected bimonthly from 2004 to 2006 at five stations. Generally, bacterial abundance was significantly higher in the summer than in the winter. The ...

The impact of diet on the gut microbiota has usually been assessed by subjecting people to the same controlled diet and thereafter following the shifts in the microbiota. In the present study, we used habitual dietary intake, clinical data, quantitative polymerase chain reaction, and denaturing gradient gel electrophoresis (DGGE) to characterize the stool microbiota of Finnish monozygotic twins...