aim : the purpose of this study was to investigate the prevalence of enteropathogenic escherichia coli (epec) and shiga toxin producing e. coli (stec) strains in healthy broilers in iran.   background : stec and epec strains as diarrheagenic e. coli are among the most prevalent causative agents in acute diarrhea. domestic animals, mainly cattle and sheep, have been implicated as the principal r...

The genetic relationship among the Escherichia coli pathotypes was investigated. We used random amplified polymorphic DNA (RAPD) data for constructing a dendrogram of 73 strains of diarrheagenic E. coli. A phylogenetic tree encompassing 15 serotypes from different pathotypes was constructed using multilocus sequence typing data. Phylogram clusters were used for validating RAPD data on the clona...

Enteroinvasive Escherichia coli (EIEC) causes dysentery; however, it is less widely reported than other etiological agents in studies of diarrhea worldwide. Between August 2003 and July 2005, stool samples were collected in case-control studies in 22 rural communities in northwestern Ecuador. Infection was assessed by PCR specific for LT and STa genes of enterotoxigenic E. coli (ETEC), the bfp ...

aim : we aimed to develop a multiplex pcr assay for specific detection of epec and ehec pathotypes based on specific marker genes. background : about 2.5 million infant’s morbidity in developing countries occurs by e.coli pathotypes because of diarrhea and intestinal diseases. the traditional phenotypic methods are time consuming and sometimes detection and differentiation of the pathotypes are...

Diarrhoeal diseases constitute one of the most important causes of illness and death all over the world. In Nigeria, the epidemiology of diarrheagenic bacteria and the virulence of the various E. coli pathotypes have not been well studied because most of the currently published data were from the southwestern axis leaving the southeast and north unstudied. 520 stool samples were collected from ...

BACKGROUND Acute diarrhea remains a major public health problem in East African nations such as Kenya. Surveillance for a broad range of enteric pathogens is necessary to accurately predict the frequency of pathogens and potential changes in antibiotic resistance patterns. METHOD Stool samples were collected from September 2009 to September 2011; 193 and 239 samples, from age-matched cases an...

background and objective: diarrheal disease is still a major health problem, especially in developing countries, where it is considered as one of the leading causes of morbidity and mortality especially in children. studies showed that diarrheagenic e. coli (dec) such as stes and epec strains are among the most prevalent causative agents in acute diarrhea, particularly in children. aim of the p...

In a study carried out in small-town and rural primary health care centers in southwestern Nigeria, 330 Escherichia coli strains isolated from 187 children with diarrhea and 144 apparently healthy controls were examined for virulence traits. Based on the results of colony blot hybridization, strains were categorized as enteropathogenic E. coli (1.8%), enterotoxigenic E. coli (2.4%), enteroinvas...

Acute diarrhea is a public health problem and an important cause of morbidity and mortality, especially in developing countries. The etiology is varied, and among pathotypes Escherichia coli are the most important.Our objectives were to determine drug susceptibility pattern to different conventional and commonly available antibiotics of diarrheagenic E. coli (DEC) in fecal samples from children...

The Port Complex of Maranhão (PCM) is the second largest port complex in Brazil, receiving ships with large volumes of ballast water. To evaluate the microbiological quality of its waters, physicochemical parameters (pH and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic Escherichia coli strains were analyzed. In order to id...