A collaborative study was undertaken at two institutions to assess the performance of a direct fluorescent-antibody stain for the detection of Pneumocystis carinii in respiratory specimens from patients with known or suspected human immunodeficiency virus type 1 infections. A total of 163 specimens (125 induced sputa, 37 bronchoalveolar lavage fluids, and 1 tracheal aspirate) from 124 patients ...

Adl2-induced tumors were homogenized and fractionated by the SCHNEIDER’S method. The CF test with the serum from tumor-bearing hamsters revealed the predominant presence of T-antigen in the mitochondrial and microsomal fractions. Hence, the rabbit was immunized with the microsomal fraction of tumors, and its serum was used to prepare the fluorescent antibody to T-antigen. The direct staining of...

In vitro cultivation of M. leprae requires a rapid, specific identification procedure for monitoring the cultures. A method utilizing direct FITC-coupled lepromatous, specific serum globulin is described in detail with suggestions for improvement. After various purification and adsorption procedures, notably against human liver powder and M. tuberculosis, a fluorescent serum preparation is obta...

The current status of the direct fluorescent antibody procedure for the diagnosis of fungus diseases is reviewed. Emphasis is placed upon those diag­ nostic applications that have been developed to a practical level. The versatility of the fluorescent antibody ( FA ) technique as a diagnostic tool in med­ ical mycology has been firmly established. It can be used for the rapid detection and iden...

Coons and Kaplan (1950) developed a rapid, sensitive, and specific fluorescent antibody technique for staining viruses in tissue sections. They used a direct staining procedure in which a specific fluorescent antibody preparation is applied directly to the cell being studied. In the indirect procedure (Weller and Coons, 1954), the virus is first reacted with a specific nonfluorescent antiserum ...

Three hundred random sputum samples were collected for analysis from cases of mild to severe respiratory infections and screened for Legionella pneumophila by both the culture method and also the direct fluorescent antigen test. In one third of the cases, blood specimen was also collected and screened for Legionella serum antibodies. With the direct fluorescent test it was possible to identify ...

purification and isolation of cellular target proteins for monoclonal antibody (mab) production is a difficult and time-consuming process. immunization of mice with murine cell lines stably transfected with genes coding for xenogenic target molecules is an alternative method for mouse immunization and mab production. here we present data on transfection efficiency of some commercial reagents us...

Immunofluorescence and fluorescent microscopy permit the demonstration of fixation of antibody, previously made fluorescent, on a specific antigen. A direct technic reveals the antigen to which globulins representing the antibodies of immune serum, previously conjugated with a fluorescent substance, are fixed. An indirect technic makes use of antihuman globulins developed in rabbit sera and mad...

The localization of Australia antigen in the liver by the fluorescent antibody technique (direct method) has been studied. Specific fluorescence was observed in the cytoplasm of liver cells. Two distribution patterns of fluorescent-positive cells, namely, a diffuse fluorescence in the liver lobules and a spotty distribution of fluorescent-positive cells, were observed.