Staphylococcus epidermidis is the most important member of coagulase negative staphylococci responsible for community and hospital acquired infections. Most clinical isolates of S. epidermidis are resistant to methicillin making these infections difficult to treat. In this study, correlation of methicillin resistance phenotype was compared with methicillin resistance (mecA) gene carriage in 55 ...

In the membrane method for measuring diffusion coefficients (Northrop and Anson (1928-29)) diffusion takes place through a porous disc. Under these conditions convection currents which usually make diffusion measurements difficult are avoided, the time required for a measurement is greatly shortened, and it becomes possible to remove the diffused substance for estimation. Under suitable conditi...

K.E.M. Hospital, Parel, Mumbai-400012. Abstract Due to cost and complexity, reference broth microdilution antifungal susceptibility method is not feasible in resource constraint setting. Therefore here is a need to develop a cost effective method which would help in simultaneous rapid identification of Candida species and performance of disc diffusion susceptibility testing. Comparison of resul...

A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the anti-tubercular drug discovery field. The fungal-SPOTi assay correlated with th...

Antibiotic sensitivities for Linezolid of 20 strains that reported as Brucella melitensis in blood cultures between 2014 and 2016 were evaluated by disc diffusion and E-test methods. According to the disc diffusion method results, 20 Brucella melitensis strains had ≥ 25 mm of antibiotic sensitivity zones. Etest measurements revealed that MIC values were between 1.5 and 6 μg. Linezolid can be an...

OBJECTIVES To compare the performance of the automated Vitek 2 system, the disc diffusion method and a home-made mupirocin screen agar (MSA) to detect mupirocin resistance in methicillin-resistant Staphylococcus aureus (MRSA) isolates. METHODS A total of 125 MRSA isolates were tested. The level of mupirocin resistance was determined by agar dilution and Etest techniques (gold standard), by th...

Many developmental systems are organised via the action of graded distributions of morphogens. In the Drosophila wing disc, for example, recent experimental evidence has shown that graded expression of the morphogen Dpp controls cell proliferation and hence disc growth. Our goal is to explore a simple model for regulation of wing growth via the Dpp gradient: we use a system of reaction-diffusio...

Ticarcillin, carbenicillin and BL-P1908, a new anti-pseudomonal semisynthetic penicillin, were subjected to several in vitro comparisons-including minimal inhibitory and lethal concentrations (MIC and MLC), effects of inoculum size, effects of buffering the media and regression analyses of MIC and disc diffusion data. Whereas ticarcillin and carbenicillin showed comparable spectra of activity, ...