rinbow trout (oncorhynchus mykiss) is an important fish species in aquaculture. the bacterial disease caused the decline in the production of this fish. lactococcus garvieae is one of the most serious infectious agents that caused disease with economical losses in fish farms. streptococosis have signs similar to lactococcosis. therefore the definite diagnosis is sometimes complicated. in the la...

Three strains of gliding bacteria resembling a known fish pathogen, Flexibacter columnaris, were recently isolated from diseased freshwater fishes in France. Morphological, physiological, and biochemical characteristics were very similar to those of 6 F. columnaris strains previously identified in other countries and used here for reference. This is the first unequivocal identification of this ...

In this study a real-time PCR assay using self-quenched primers labelled with a single fluorophore for the detection of Aeromonas salmonicida was developed. Probe specificity was confirmed by amplification of 16 A. salmonicida strain templates and by the lack of a PCR product with 26 non-A. salmonicida strains. With a pure culture of A. salmonicida, the assay was linear over a range of 0.5 pg t...

We report here the first description in North Portugal of epizootic episodes of lactococcosis causing important mortalities in cultured rainbow trout associated with high water temperatures. The outbreaks that occurred during the warm seasons of 2002 and 2003 affected all size fish, reaching up to 90% mortality in some farms. The taxonomic studies of the isolates obtained indicated that they be...

BACKGROUND There have been an increasing number of infections in fish associated with different species of Chryseobacterium, being considered potentially emerging pathogens. Nevertheless the knowledge of the diversity of species associated with fish disease is partial due to the problems for a correct identification at the species level based exclusively on phenotypic laboratory methods. RESU...

A comparative analysis of the microbiological quality of three turbot (Scophthalnus maximus) farms (A, B, and C) located in Galicia (northwest Spain) is given. The microbial load and types of bacteria in the internal organs (liver and kidney) of apparently healthy fish was monitored over a year, and all the disease problems occurring during this survey were analyzed. The percentage of healthy t...

We determined the susceptibilities of 144 clinical and 49 environmental Aeromonas strains representing 10 different species to 26 antimicrobial agents by the agar dilution method. No single species had a predominantly nonsusceptible phenotype. A multidrug nonsusceptible pattern was observed in three (2.1%) clinical strains and two (4.0%) strains recovered from diseased fish. Common clinical str...

Vibrio harveyi is one of the major pathogens in aquaculture. To identify key virulence factors affecting pathogenesis V. towards fish, we conducted a field investigation for three representative fish farms infected with harveyi. Multilocus sequence typing (MLST) and whole-genome sequencing were to delineate phylogenetic relationship genetic divergence A total 25 strains isolated from diseased g...

eight medicinal plants were assessed for antimicrobial activity against lactococcus garvieae isolate obtained from diseased oncorhynchus mykiss collected from rainbow trout fish farms in iran. lactococcus garvieae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems. the antibacterial activity of the medicinal p...

Tilapia tilapinevirus or tilapia lake virus (TiLV) is an emerging that inflicts significant mortality on farmed globally. Previous studies reported detection of the in multiple organs infected fish; however, little known about in-depth localization central nervous system. Herein, we determined distribution TiLV entire brain experimentally Nile tilapia. In situ hybridization (ISH) using TiLV-spe...