AbstractMolecular identification using short orthological DNA sequences (DNA barcoding) has been applied for the classification of orchid species which is a major step in biodiversity management, conservation, breeding, authenticating components herbal products, and tracking adulteration species. One most widely used loci phylogenetic inference at generic infrageneric levels plants was ITS loca...

In the past decades, the use of traditional medicine has increased globally, leading to a booming herbal medicine and dietary supplement industry. The increased popularity of herbal products has led to a rise in demand for botanical raw materials. Accurate identification of medicinal herbs is a legal requirement in most countries and prerequisite for delivering a quality product that meets cons...

The genus Berchemia comprises important Chinese plants with considerable medicinal value; however, these plants are often misidentified in the herbal medicinal market. To differentiate the various morphotypes of Berchemia species, a proficient method employing the screening of universal DNA barcodes was used in this work. Three candidate barcoding loci, namely, psbA-trnH, rbcL, and the second i...

Timely and accurate biodiversity analysis poses an ongoing challenge for the success of biomonitoring programs. Morphology-based identification of bioindicator taxa is time consuming, and rarely supports species-level resolution especially for immature life stages. Much work has been done in the past decade to develop alternative approaches for biodiversity analysis using DNA sequence-based app...

Microalgae identification is extremely difficult. The efficiency of DNA barcoding in microalgae identification involves ideal gene markers and approaches employed, which however, is still under the way. Although Scenedesmus has obtained much research in producing lipids its identification is difficult. Here we present a comprehensive coalescent, distance and character-based DNA barcoding for 11...

In the edible mushroom market, porcini (Boletus edulis Bull., and related species) are a very economically valuable product, with 20–100 tons of these mushrooms being sold each year worldwide. However, products as “Boletus edulis” often mix different species, some them less value than but at same price. DNA-Barcoding is powerful tool that can help uncover this kind fraud in rapid accurate way. ...

Elliott, T.L. (corresponding author, [email protected] ), Waterway, M.J. ([email protected]), Davies, T.J. ([email protected]) Department of Biology, McGill University, 1205 Docteur Penfield Avenue, Montreal, QC H3A 1B1, Canada; Department of Plant Science, McGill University, 21 111 Lakeshore Road, Ste-Annede-Bellevue, QC H9X 3V9, Canada; African Centre for DNA Barcoding, Un...

BACKGROUND Identification keys are decision trees which require the observation of one or more morphological characters of an organism at each step of the process. While modern digital keys can overcome several constraints of classical paper-printed keys, their performance is not error-free. Moreover, identification cannot be always achieved when a specimen lacks some morphological features (i....