Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of horseradish peroxidase. First, an oxidized PSi optical nanostructure, a Fabry-Pérot thin film, is synthesized and is used as the optical transducer element. Immobilization of the enzyme onto the nanostructure is performed through DNA-directed immobilization. Preliminary studies demonstrate high enzyma...

Two new models for implementing finite state machines with DNA computing are presented. The operations used in both models are simple and easy to implement. Operations include immobilization of DNA strands onto paramagnetic beads, DNA hybridization, DNA ligation and restriction enzyme cleavage. Use of paramagnetic beads greatly reduces performance time and demonstrates DNA chip compatibility of...

Polyacetylene vesicle has attracted attentions due to its unique chromatic properties, such as blue-to-red transition. The liposome-like vesicle was immobilized on solid substrates to detect small quantity analytes. In this case, fluorescence was used for detection. We successfully detected protein and bacteria using antibodies or aptamers on the vesicles as probes. Recently, we developed a dif...

The conformation of a three-dimensional polymeric coating (copoly(DMA-NAS-MAPS)) and immobilization and hybridization of DNA strands on the polymer coated surface are investigated. A conformational change, specifically the swelling of the surface adsorbed polymer upon hydration, is quantified in conjunction with the application of this polymer coating for DNA microarray applications. Fluorescen...

DNA origami structures represent an exciting class of materials for use in a wide range biotechnological applications. This study reports the design, production, and characterization “zipper” structure, which contains nine pH-responsive locks. Each lock consists two parts that are attached to zipper’s opposite arms: hairpin single-stranded able form triplex through Hoogsteen base pairing. The s...

In a previous study (Kelleher AR, Kimball SR, Dennis MD, Schilder RJ, and Jefferson LS. Am J Physiol Endocrinol Metab 304: E229-236, 2013.), we observed a rapid (i.e., 1-3 days) immobilization-induced repression of mechanistic target of rapamycin complex 1 (mTORC1) signaling in hindlimb skeletal muscle of young (2-mo-old) rats that was associated with elevated expression of regulated in develop...

The capsular polysaccharide (CPS) is the most important identified virulence factor of Streptococcus pneumoniae, a human pathogen of the upper respiratory tract. One limitation in studies of S. pneumoniae surface virulence factors is the lack of a reliable procedure for isolation of capsule-negative mutants of clinical strains. This paper presents an approach, based on the immobilization of pne...