SV40 (simian virus 40)-infected CV1 cells were permeabilized with Staphylococcus aureus α-toxin for small molecules (<2 kDa) in a medium that supports DNA replication. Incorporation of [α-P]dATP was shown to proceed at an essentially constant rate for at least 1 h. P-labelled DNA replication intermediates and products were analysed by alkaline sucrose density centrifugation. The results suggest...

With the exception of imprinted genes and certain repeats, DNA methylation is globally erased during preimplantation development. Recent studies have suggested that Tet3-mediated oxidation of 5-methylcytosine (5mC) and DNA replication-dependent dilution both contribute to global paternal DNA demethylation, but demethylation of the maternal genome occurs via replication. Here we present genome-s...

Although HCMV encodes many genes required for the replication of its DNA genome, no HCMV-encoded orthologue of the origin binding protein, which has been identified in other herpesviruses, has been identified. This has led to speculation that HCMV may use other viral proteins or possibly cellular factors for the initiation of DNA synthesis. It is also unclear whether cellular replication factor...

In 1954 Max Delbrück published "On the Replication of Desoxyribonucleic Acid (DNA)" to question the semi-conservative DNA replication mechanism proposed that James Watson [2] and Francis Crick [3] had proposed in 1953. In his article published in the Proceedings of the National Academy of Sciences [4], Delbrück offers an alternative DNA replication mechanism, later called dispersive replication...

DNA replication is a key process in cell division cycle. It is initiated in coordinated manner in several species. To understand the DNA replication in a species one needs to measure the half replication timing (or replication timing) and the efficiency of replication which vary across genome in higher eukaryotes. In the previous studies, no direct assessment of replication efficiency on a geno...

Proper control of DNA replication is essential to ensure faithful transmission of genetic material and prevent chromosomal aberrations that can drive cancer progression and developmental disorders. DNA replication is regulated primarily at the level of initiation and is under strict cell-cycle regulation. Importantly, DNA replication is highly influenced by developmental cues. In Drosophila, sp...

Faithful DNA replication ensures genetic integrity in eukaryotic cells, but it is still obscure how replication is organized in space and time within the nucleus. Using timelapse microscopy, we have developed a new assay to analyze the dynamics of DNA replication both spatially and temporally in individual Saccharomyces cerevisiae cells. This allowed us to visualize replication factories, nucle...

The intracellular replication of MVL51, a group L1 mycoplasmavirus, was investigated. The single-stranded parental DNA was found to enter the cell and become converted to double-stranded DNA. This replicated to yield additional double-stranded DNA molecules. The parental viral DNA was found to leave the replication complex and become associated with large molecular weight DNA not involved with ...