PURPOSE To describe the technique of limbal stem cell-sparing corneoscleroplasty for the management of advanced keratoglobus. METHODS A patient with bilateral advanced keratoglobus, with best-corrected visual acuity of 20/400 in the right eye and 20/200 in the left eye, underwent limbal stem cell-sparing corneoscleroplasty of the right eye. Initially, a 360-degree limbal incision with 200-μm ...

PURPOSE To determine the effects of proinflammatory cytokines on differential gene expression profiles in the human corneal endothelium (HCE), by using a cDNA expression array. METHODS A human cDNA expression array technology was used to study the simultaneous expression of HCE incubated with interleukin (IL)-1alpha and tumor necrosis factor-(TNF)-alpha. Gene-specific semiquantitative reverse...

PURPOSE To characterize quantitatively the paracellular permeation routes in rabbit cornea, conjunctiva, and sclera using polyethylene glycol (PEG) oligomers. METHODS Corneal, conjunctival, and scleral tissues from New Zealand white rabbits were tested individually in a modified two-chamber Ussing apparatus with the mixture of PEGs with mean molecular weights 200, 400, 600, and 1000 in glutat...

PURPOSE To demonstrate relationship between herpes simplex virus (HSV) corneal latency and graft survival. METHODS Prospective case control study. 28 recipient corneal buttons and donor cornea-scleral remnants were examined for HSV DNA with polymerase chain reaction (PCR). None of the recipient had a history of HSV infection. Serum samples of graft recipients were analyzed for the presence of...

PURPOSE To determine the immunogenic characterization of amniotic epithelium (AE), by examining the fate of allogeneic AE grafts heterotopically transplanted in the eye. METHODS Intact AE from enhanced green fluorescence protein (EGFP) transgenic mice (C57BL/6 background) and wild-type C57BL/6 mice were transplanted onto cornea or conjunctiva, or inserted into the anterior chamber (AC) of nor...

lar to the earlier described case, suggesting an invasive postoperative ingrowth process. The X-Y karyotyping revealed that the epithelial cells were of donor origin. The technique of posterior lamellar keratoplasty is relatively new and technically difficult, with a surgeon’s learning curve. The most critical step is the preparation of the posterior lamellar disc by hand or by use of a microke...

purpose : to compare the outcome of surgery and postoperative complications between using manually dissected cornea in descemet’s stripping with endothelial keratoplasty (dsek) and using eye bank automated precut cornea in descemet’s stripping with automated endothelial keratoplasty (dsaek) methods : forty eyes with indication of endothelial keratoplasty (ek) were included in this randomized pr...

Background: The present study was conducted to the donor profile and assess trends of cornea donation. Methodology: This as a hospital based cross sectional at tertiary care centre in Central India for period 5 years. Data retrieved from 70 patients eye bank who filled form donation Eye our institution. Sociodemographic donors, cause death time since recorded. Source information regarding corne...

purpose: to evaluate bacterial and fungal contamination of donor corneas before and after decontamination at eye bank of i.r. of iran based on cultures. methods: this descriptive study was performed on 131 donor eyes (whole globe or cut cornea) used for corneal transplantation at labbafinejad hospital during a 6-month period. microbiologic culture was performed at three stages: before enucleati...

Introduction: Keratoconus is a relatively common disease of cornea in which structural changes within the cornea cause it to thin and change to a conical shape and scar at the central portion of cornea. So far, few methods and drug treatments were introduced due to both lack of accepted animal models to induce experimental keratoconus and limitation of research in human considering ethical issu...