Five subtypes of dopamine receptor exist in two subfamilies: two D1-like (D1 and D5) and three D2-like (D2, D3 and D4). We produced novel monoclonal antibodies against all three D2-like receptors and used them to localize receptors in Ntera-2 (NT-2) cells, the human neuronal precursor cell line. Most of the immunostaining for all three receptors colocalized with mannose-6-phosphate receptor, a ...

Mesolimbic dopamine controls drug and alcohol seeking behavior. Stimulation of dopamine D3 autoreceptor reduces extracellular levels of dopamine. We tested the hypothesis that dopamine D3 receptor (D3R) gene deletion or its pharmacological blockade counteracts alcohol preference and intake in a long-term voluntary ethanol intake paradigm. Mice D3R -/and their wild type (WT) littermates, treated...

Despite extensive study, the G protein coupling of dopamine D3 receptors is poorly understood. In this study, we used guanosine-5'-O-(3-[35S]thio)-triphosphate ([35S]-GTPgammaS) binding to investigate the activation of G proteins coupled to human (h) D3 receptors stably expressed in Chinese hamster ovary (CHO) cells. Although the receptor expression level was high (15 pmol/mg), dopamine only st...

None of the receptor ligands studied: the dopamine D2 antagonist eticlopride, D2/D3 antagonist haloperidol, alpha1-adrenergic antagonist prazosin, N-methyl-D-aspartate antagonist MK-801, 5-HT2/D2 antagonist mianserin, D2/D3 agonist quinpirole and alpha2-adrenergic agonist clonidine co-administrated with repeated amphetamine (AMPH) injections blocked the AMPH-induced decrease in the striatal and...

Recently, zinc has been shown to modulate antagonist drug interactions with the D1 dopamine receptor (Schetz and Sibley, 1997) and the dopamine transporter (Norregaard et al., 1998). We now demonstrate that zinc also reversibly and dose-dependently modulates the specific binding of the butyrophenone antagonist [3H]methylspiperone to all D2-like dopamine receptors: D2L, D3, and D4. The molecular...

In vivo administration or in vitro application of dopamine or of dopamine receptor agonists induce vasodilatation in the cerebral, coronary, renal and mesenteric vascular beds and cause hypotension. Moreover, dopamine stimulates cardiac contractility and induces diuresis and natriuresis. Peripheral (cardiovascular and renal) dopamine receptors belong to the D1-like and D2-like receptor superfam...