Directed evolution relies on iterative cycles of randomization and selection. The outcome of an artificial evolution experiment is crucially dependent on (i) the numbers of variants that can be screened and (ii) the quality of the assessment of each clone that forms the basis for selection. Compartmentalization of screening assays in water-in-oil emulsion droplets provides an opportunity to scr...

All-Natural Pickering Double Emulsions A facile and green one-step emulsification approach using oppositely charged binary bio-based particles to readily prepare all-natural double emulsion is presented in article number 2101568 by Yunxing Li, Cheng Yang, To Ngai, co-workers. The formation of attributed the suitable wettability aggregates formed phase inversion during preparation. Furthermore, ...

We have obtained nearly one thousand events of the Ξ− hyperon captured at rest in nuclear emulsion. Among them, production and decay of double-Λ hypernuclei was successfully found and analyzed for five events which correspond to five nuclear species with double strangeness. Preliminary results are discussed for the binding energy and interaction energy of two Λ hyperons. By comparison with olde...

Water-in-oil emulsions are commonly used to provide persistent release of antigens necessary for sustained stimulation of the immune system. Optimal response of an animal's immune system is dependent upon repeated, maintained exposure to the antigen. The sustained release of antigen, which delays catab-olism, is thought to be one of the major methods by which adjuvants enhance the immune respon...

In this study, the potential of PTMC and mPEG-PTMC microparticles for controlled delivery of hydrophilic drugs was investigated. PTMC and mPEG-PTMC microparticles, loaded with hydrophilic model compounds (BSA, lysozyme and CoomassieR Brilliant Blue G) were prepared by the double emulsion method. High loading efficiencies can be achieved, and first order release profiles during 60 days were obse...

We demonstrate a microfluidic method to first generate double emulsion droplets containing two different inner drops, and to then control the internal coalescence of the encapsulated drops. The advantages of the core-coalescence method are illustrated by fabricating high viscosity particles and by demonstrating the dissolution of cell membranes.

Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed.

We present a simple microfluidics-based technique to fabricate Janus particles using double-emulsion droplets as templates. Since each half of the particles is templated from a different immiscible fluid, this method enables the formation of particles from two materials with vastly different properties. The use of microfluidics affords excellent control over the size, morphology, and monodisper...

“Pitanga” leaf hydroethanolic extract (PLHE) presents high antioxidant and antimicrobial activities. The encapsulation of this in W/O/W double emulsions could protect its bioactive properties. objective research was to encapsulate PLHE the inner W phase a emulsion analyze emulsifiers concentrations ratios effects on droplet size physical stability. Moreover, rheological behavior, activities the...

A reliable microfluidic platform for the generation of stable and monodisperse multistage drug delivery systems is reported. A glass-capillary flow-focusing droplet generation device was used to encapsulate thermally hydrocarbonized porous silicon (PSi) microparticles into the aqueous cores of double emulsion drops, yielding the formation of a multistage PSi-lipid vesicle. This composite system...